Biochem 2 Flashcards
1
Q
Restriction enzymes
A
PALINDROMIC
5’ to 3’ same in both strands
2
Q
cDNA
A
- only coding regions (Vs genomic library) from mRNA
- recombinant proteins
3
Q
recombinant proteins are from
A
cDNA
4
Q
lactose
A
galactose-B-1,4-glucose
5
Q
What type of inhibition can be overcome by excess substrate?
A
Competitive
Noncompetitive binds equally to E and E-S
Uncomp solely E-S
6
Q
Antiaromatic
A
- unstable/reactive
- cyclic, PLANAR, conjugated
- 4n (add 2 for aromatic)
7
Q
Pressure / moles of gas equilbrium
A
-increasing pressure shifts rxn towards fewer moles of gas
8
Q
Doing what to blood vessels increases oxyhemoglobin?
A
vasoconstriction
Hb + O2 -> HbO2
Shifting to fewer moles of gas = increase pressure = constrict
9
Q
intrinsic factors bind B12 better in what kind of environment?
A
alkaline
10
Q
postprandial state
A
well-fed state
nutrients flow through liver first via hepatic portal vein
lasts 3-5 hours after meal
marked by anabolism + fuel storage
