BIO3 Flashcards
BIO3
What determines protein folding?
Favorable and unfavorable interactions influence folding energy.
What are intrinsically disordered proteins?
Proteins that lack a well-
defined structure - associated with signaling and regulatory processes.
What are examples of protein misfolding/aggregation diseases?
Examples include Alzheimer’s and Parkinson’s diseases.
What is allostery?
Process where a a molecule binds to a protein at one site, causing a change in the protein’s shape and activity at a different site.
What are enzymes and why are they superior to non-biological catalysts?
Enzymes are biological catalysts with high specificity and efficiency.
What is directed evolution and how is it used to improve enzymes?
Directed evolution is a method for evolving enzyme functionality.
What are antibodies and their roles in biology and applications?
Antibodies recognize foreign substances, essential in immunity and therapies.
What are nanobodies, and why are they better suited for some applications?
Nanobodies are smaller, more stable, and easier to produce than antibodies.
What is the key idea of protein display technologies like phage display?
Display technologies present proteins like antibodies on the surface of viruses or other platforms.
What is proteomics and what experimental method is it based on?
Proteomics studies the protein set of a cell, often via mass spectrometry.
Advantages and disadvantages of chemical synthesis vs recombinant production of proteins?
Chemical synthesis is faster, higher error rater; recombinant is more scalable more accurate folding.
What is self-assembly, and what is its role in biology?
Molecules adopt a defined arrangement without guidance. Also called “folding”.
Examples of sustainable sourcing of food proteins.
Examples include plant-based and lab-grown protein sources.
