Bio Lab Midterms

Question 1

Capable of Hydrolizing peptide bonds

Answer 1

Proteolytic enymes

Question 2

Peptidases/proteases or protinases are

Answer 2

Enzymes

Question 3

Action of proteolytic enzyme

Answer 3

Digestion of proteins
Protein turnover
Cell division
Blood clotting cascade
Signal transduction
Processing of polypeptide hormones
Apoptosis

Question 4

cell death of several disease causing organims(viruses)

Answer 4

Apoptosis

Question 5

Generic name given to set of derived endopeptides from bromeliaceae

Answer 5

Bromelain

Question 6

Ananas comosus

Answer 6

Pineaple

Question 7

Function of bromelain

Answer 7

Break peptide bond
Separates protein and amino acids

Question 8

Optimum temp and pH of bromelain

Answer 8

50c and 7.0pH

Question 9

Physical changes due to being exposed to abnormal conditions

Answer 9

Denaturation

Question 10

Factors of protein denaturation

Answer 10

Radiation
Extreme pH
Organic solvent
High salt concentration
Mechanical agitation

Question 11

Ninhydrin test

Answer 11

For free amino acids
Ninhydrin reagent
Violet

Question 12

Biuret test

Answer 12

For peptide bonds
Cupric sulfate in sodium hydroxide
Violet

Question 13

Xanthoproteic test

Answer 13

For aromatic amino acids (Phe, Trp,Tyr)
Concentrated Nitric acid
Yellow

Question 14

Millons test

Answer 14

For phenolic a.a Tyr
Mercury in Nitric acid
Rose/salmon

Question 15

Hopkins cole test

Answer 15

For indole ring Trp
Glyoxylic acid in Sulfuric acid
Violet ring

Question 16

Sakaguchi test

Answer 16

For guanidine Arg
10% alpha-naphtol in NaBro/NaCIO
Reddish wine

Question 17

Nitroprusside test

Answer 17

For thiol cysteine
Sodium nitroprusside
Red

Question 18

Fohls test

Answer 18

Sulfur Cys Met
Lead II acetate
Brown or black

Question 19

Amide test

Answer 19

For amide Asn,Gln
sodium hydroxide+heat
Litmus paper red to blue or blue to blue

Question 20

Paulys test

Answer 20

For phenolic/imidazole (Tyr,His)
Diazotized sulfanilic acid
Red