BIO CHEM & BIO Flashcards
In animals do we have L or D amino acids? Sugars?
L amino acids and D sugers
Proteases usually have which aa in their active cite? Why?
Serine, OH group act as a nucleophile
Recognition pcoket
pocket in the enzyme which attracts certain residues on substrate polypeptides.
Cofactors
metal ions or small molecules requireed for activity of enzyme
Coenzyme
a subgroup of cofactors that are organic
covalent modification happens most often to which aa? by which enzyme?
ser, the, tyr by kinase to switch OH with PO4
Kinase vs phosphorylase?
Kinase use P from organic, Phosphorylase use P from floating inorganic P
Phosphatase is the opposite of which enzymes (2)?
Kinase (from ATP) and phosphorylases (from inorganic P)
Zymogen?
Inactive protien that needs to have a haircut
Ways of regulating enzymes (4)?
Covalent modification
proteolytic cleavage
association with other polypeptides
allosteric regulation
Feedback inhibition vs feedback stimulation vs feedforward stimulation
page 85 Bio chem
If there is only a little substrate, then the rate V is …
directly proportional to the amount of substrate (i.e. linear)
What is the difference between allosteric and cooperative enzymes?
Cooperativity takes place at an active site which acts as an allosteric site as well (i.e. specail case of allosteric). While other allosteric happen just on an allosteric site.
Compatitve inhibitor Vmax & Km? Site? molecule (free or complex enzyme?)
Non- comp?
Un comp?
Mixed ?
Comp = same Vmax increase Km, active site, free enz
Non comp = decrease Vmax same Km, allosteric, free
Un comp = decrease Vmax increase Km, allosteric, complex
Mixed = decrease Vmax Km varies, allosteric, both free and complex (if more affinity to free enzyme = like comp inhib, if affinity for complex = like uncomp),
Lineweaver Burk Plot
what is the y-intercept
x-intercept?
How un/non/and comp inhibitors act on LBP?
y = 1/vmax x = 1/-km non comp = higher y same x un comp = higher y and x comp = same y smaller x
Gibbs free energy equation
4G=4H-T4S
First law of thermo
2nd?
1st = energy conservation 2nd = entropy increase
Keq equation?
[c][d]/[a][b]
Dalta G’ equation
G’ = -RT InKeq
The higher the Ka the xxxxxxxxxx the acid
stronger
Ka equation
Ka = [H3O+][A-]/[HA]
high pka means?
Weak acid
polyprotic vs amphoteric?
poly protic = multiple hydro to lose as acid
amphoteric = can be acid twice
ph =
ph= -log[h+]
pka =
pka = -log [ka]
aa? R = CH2COOH
Aspartic acid
aa? R= CH2CH2COOH
Glutamic acid (other name for deprotonated?)
Lysine structure? one letter?
c-c-c-c-N
K
Arginine Structure? one letter
c-c-c-n-c(2N)
R
Histidine structure? One letter?
c-ring with NH
h
Glycine
r = H
G
Alanine?
A
CH3
valine
V carbons
V
Leucine?
L Y carbons (4 c total)
Isoleucine?
Sowrd carbons C(C)-C-C
I
Phenylalanine?
C-benzene ring
F
Tryptophan?
C-RINGRING
W
Serine?
C-OH
S
Threonine?
T
C(C)-OH
Tyrosine?
Y
C-Benzene ring- OH
Asparagine?
CC=O/-N
N
Glutamine
C-C-C=O/-N
Q
Cysteine?
C
c-s
Methionine
C-C-S-C
M
Essential aa?
Valine Phen “Hiss” and Lys Isolate Leu (the hero) who Met Three Trys (to defeat phen)
Val his leu iso phen try met lys thr
C-C name?
C C name?
cystine (bond)
cysteine (no bond)
Hydrolase?
Hydrolyzes chemical bonds (ATPases, proteases, etc)
Isomerase?
rearrange to make isomers
Ligase?
form chemcial bonds (DNA ligase) i.e. glue
lyase?
break bonds by ways other than oxidation or hydrolysis (e.g. pyruvate decarboxulase)
Kinase?
Put P on a molecule from ATP
oxidoreductase?
Runs redox
polymerase?
polymerization (DNA polymerase)
phosphatase?
remove P group from molecule
phosphorylase?
put P on molecule from inorganic (floating) P
protease?
hydrolyzes peptide bonds
nucleons
protons + neutrons
atomic number Z?
Number of protons
Mass number A
Number of Nutrons + protons
strong nuclear force works where?
In the nucleus. holding together strong protons and neutrons.
can you tell what is radioactive nuclei, radioactive decay, parent and daoughter nuclei?
No? Go to page 53 u dump bitch
alpha particle made of?
2 proton and 2 nuotrons
Where is waek nuclear force found (decay wise)
Beta decay
What are the three types of Beta decay?
B- B+ and electron capture
what is positron?
positive electron