Bio 130 Flashcards
STR
Single tandem repeat. Helps differentiate between people’s DNA.
(TTTTTT vs TTTTTTTTTTTTTT)
Homology
Similar traits that come from common ancestor
Analogy
Similar traits that develop separately
Proto-oncogene
regulate cell growth. gene that if TURNED ON leads to cancer
Tumor suppressor genes
regulate cell growth, brakes on cell growth that leads to cancer. genes that if TURNED OFF leads to cancer
Telomere
shortens with each DNA synthesis, causing aging. if telomerase is TURNED ON in non-gonadal cells it leads to cancer
Contact Inhibition
Cells cease growth if they touch each other. if TURNED OFF causes cancer
Anchorage Dependence
requires normal cells to attatch to a solid surface tospread and grow, if TURNED OFF causes cancer
Chargaff’s Rule
Equal # of A & T. Also G & C
Law of Segregation
each gametes contains only a single allele of any given gene
Law of Independent Assortment
alleles of different genes should be passed on independent of each other
Linked Genes
Exceptions to Law of Independent Assortment. Genes close to each other on chromosome often get inherited together
Helicase
“unzips” 2 strands of DNA by breaking H bonds
DNA Polymerase
“builder”, replicates DNA molecules to build new strand of DNA
Primase
“initializer”, makes primers so DNA Polymerase can figure out where to start
Ligase
“glues” DNA fragments together
PCR
method used to make a ton of copies of a specific DNA sample
qPCR
monitors amplification of targeted DNA molecules during PCR. Measures in increases in florescent signals
Western Blot
detects specific PROTEINS in sample by creating antibodies that bind to the protein and show up on the test
Northern Blot
study gene expression by detection of RNA in sample. can see if something’s off in patients by unusual amounts of RNA
Nondisjunction
happens in Anaphase. Chromosomes/chromatids separate unequally
incomplete dominance
dominant and recessive, but dominant cant take over completely. creates new phenotype (ex: red + white= pink)
Codominance
2 dominant. both take up dominance. (ex: spotted pink and white flower)
Ploidy Number
number of copies of chromosomes (2n, 3n, etc.)
mRNA splicing and processing
remove introns, splice exons together
Codon
3 nucleotides in mRNA, code for amino acid
Anticodon
3 nucleotides in tRNA, pair to codon
missense mutation
nucleotide replaced, amino acid changes
frameshift mutation
nucleotide is either inserted or deleted. Everything after is shifted
silent mutation
nucleotide replaced, codes for same amino acid though
nonsense mutation
nucleotide replaced, codes to stop
What charge is DNA
negative
Calorie
amount of energy required to raise temp of 1 gram of water by 1 degree Celius
Calorie formula
(water mass)(temp change)/(mass of food burned)
Carbs
provide energy, some structural
Proteins
function and structure
lipid
store energy
Nucleic acid
build DNA
Isotope
same element, different masses
Macromolecules
Protein, Lipids, Carbohydrates, Nucleic Acids
Saturated fat
single-stranded bonds, causes it to stack and be able to be solid at room temperature
Unsaturated fat
double-stranded bonds, can’t stack because of bend, liquid at room temperature
Covalent Bond
sharing electrons
Hydrophilic particles
attracted to water. charged, ionic, polar
Hydrophobic particles
repelled by water. noncharged, non-polar
If an element is on a far side of the elemental table it is more likely to create an…
ionic bond
ionic bonds
transfer of electrons (ex: NaCl)
Adhesion
water molecules are attracted to other surfaces
Cohesion
water molecules are attracted to each other
High surface tension
hydrogen bonds are strong enough to hold certain objects with small mass without bonds breaking
High standard heat
since hydrogen bonds are harder to break, it takes more energy to change the state of water
Enzymes
proteins that catalyze reactions by lowering their activation energy
Exergonic reaction
energy releasing
Endergonic reaction
energy absorbing
Passive Transport
energy-free/ natural movement of molecules. High to low conc. (facilitated, simple)
Facilitated Diffusion
diffusion happens naturally if provided a channel to move across
Simple Diffusion
only small, nonpolar molecules can cross membrane without help
Active Transport
requires energy to move molecules across. Often moves against conc. gradient (not all the time though)
Hypotonic environment
environment has less solute. water goes into cell.
Hypertonic environment
environment has more solute. water comes out of cell
Isotonic environment
environment and cell have same levels of solute
Fluid-mosaic model
membranes are fluid, things can move in them and are constantly changing
Desmosome Junction
connect cytoskeletons to cell for cell communication
Tight Junction
seal in cells for cell communication
Gap Junction
channel between cells for cell communication
3 stages of Cellular Respiration
Glycolysis, Citric Acid Cycle, Electron Transport Chain
Cellular Respiration
breaking down stored energy in sugar to make useful energy in form of ATP
Glycolysis
breaking down glucose to make Pyruvate (also get NADH and ATP)
Citric Acid Cycle
takes carbon chain. Outputs: NADH, ATP, CO2
Electron Transport Chain
Uses elections to transport H across conc. gradient
Photosynthesis
energy from sun to create glucose