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Bio 110 Lab > Bio 110 Lab 6 > Flashcards

Bio 110 Lab 6 Flashcards

(75 cards)

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1
Q

light microscopes use

A

light rays passing thru lenses to magnify the object.

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2
Q

stereomicroscope is also called the

A

dissecting microscope

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3
Q

the steromicroscope is designed to study the

A

entire object in 3D at low magnification

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4
Q

the compound microscope is used for examining

A

small or thinly sliced sections of objects under higher magnification than the stereomicroscope.

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5
Q

the term compound in compound microscope refers to

A

the use of two sets of lenses.

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6
Q

the 2 sets of lenses in a compound microscope are:

A

the ocular lenses and the objective lenses

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7
Q

illumination in a compound microscope is from

A

below.

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8
Q

in a compound light microscope light

A

is from below, then visible light passes thru clear portion of the specimen, but does not pass thru opaque portions.

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9
Q

to improve contrast with a light microscope

A

stains or dyes that bind to cellular structures and absorb light is used

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10
Q

photomicrographs or light micrographs

A

are images produced by a compound light microscope

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11
Q

electron microscopes use

A

beams of electrons focused on a photographic plate by means of electromagnets to magnify the object

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12
Q

transmission electron microscope is comparative to

A

the compound light microscope

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13
Q

the object viewed with a TEM is

A

ultra-thinly sliced and treated with heavy metal salts to improve contrast.

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14
Q

the scanning electron microscope is comparative to

A

the dissecting light microscope

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15
Q

the object viewed with a SEM is

A

an image of the surface and dimensions of an object

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16
Q

the EM has a greater

A

resolving power

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17
Q

resolution is

A

the minimum distance between 2 objects at which they can still be seen as 2 separate objects.

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18
Q

what gives a much greater resolving power

A

the use of high energy electrons

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19
Q

CLM have _____ lenses

TEM have ______ lenses

A

glass

electromagnetic

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20
Q

CLM illumination is by

TEM illumination is by

A

visible light

beam of electrons

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21
Q

CLM resolution is approx.

TEM resolution is approx.

A

200 nm

0.1 nm

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22
Q

CLM magnifies to

TEM magnifies to

A

2,000x

1,000,000x

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23
Q

CLM costs up to

TEM costs up to

A

tens of thousands

hundreds of thousands

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24
Q

the stereomicroscope allows you to

A

view objects in 3D at low magnifications.

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25
the stereomicroscope is used to study
entire small organisms, any object requiring lower magnification, and opaque objects that can be viewed only by reflected light. called a stereomicroscope because it produces a 3D image.
26
binocular head
(DS)holds 2 eyepiece lenses that move to accommodate for the various different individuals' eyes
27
eyepiece lenses
(DS)the 2 lenses located on the binocular head
28
independent focusing eyepiece | fixed eyepiece
(DS) some models have and a knob to allow independent adjustment of each eye. non adjustable eyepiece
29
focusing knob
(DS) knob located on the arm, used for changing focus of both eyepieces together.
30
magnification changing knob
(DS) often built into the binocular head, used to change the magnification in both eyepieces simultaneously. may be a zoom mechanism or a rotating lens mechanism of different powers that clicks into place.
31
illumination
(DS) used to illuminate an object from above; may be built into microscope or separate.
32
CLM is used to examine
small or thinly sliced sections of objects under higher magnification than would be possible with the stereomicroscope.
33
with a CLM light is
from below and passes thru the clear portions but does not pass thru the opaque portions.
34
eyepieces (CLM)
ocular lenses (10x)
35
viewing head (CLM)
holds ocular lenses
36
arm (CLM)
supports upper parts and provides carrying handle
37
nosepiece (CLM)
revolving device that holds objectives
38
objectives (CLM)
objective lenses (ones closest to specimen)
39
scanning objective (CLM)
shortest of objective lenses and is used to scan the whole slide. (4x)
40
low power objective (CLM)
longer than scanning objective and is used to view objects in greater detail than scanning objective(10x)
41
high power objective (CLM)
used to view objects in greater detail than low power (40x)
42
oil immersion objective (CLM)
used in conjunction with immersion oil to view objects with the greatest magnification. (100x)
43
stage (CLM)
platform that holds and supports microscope slides..
44
mechanical stage (CLM)
moveable stage that aids in the accurate positioning of the slide.
45
stage clips (CLM)
clips that hold a slide in place on the stage
46
mechanical stage control knobs (CLM)
2 knobs that control forward/reverse movement and right/left movement
47
coarse adjustment knob (CLM)
knob used to bring object into approximate focus; used only with low power objective
48
fine adjustment knob (CLM)
used to bring object into final focus
49
diaphragm or diaphragm control lever (CLM)
lever that controls the amount of light passing thru the condenser.
50
light source (illuminator) (CLM)
attached lamp that directs a beam of light up thru the object
51
base (CLM)
flat surface of the microscope that rests on the table.
52
inversion
image is flipped upside down and reversed
53
the ocular lens has a magnification of
10x
54
parafocal
once the object is in focus with low power it should be almost in focus on the higher ones
55
total magnification is calculated by
ocular lens x objective lens
56
field of view
circle visible thru the lenses
57
diameter of field
length of the field from one edge to the other.
58
HPD=
LPD X LPM/HPM
59
which power has a larger field of view
low power
60
which power has a smaller field of view but a greater magnification
high power
61
to locate small objects on a slide
find them on scanning, put them in the center of the field then rotate to low power
62
the depth of field
is the area-from top to bottom that comes into focus when slowly focusing with the fine adjustment knob
63
when one thread is in focus
the others are blurred
64
as the stage moves upward or objectives move downward
objects on top come into focus first
65
the depth of field is more shallow on
high power.
66
focusing up and down will give you an idea of
the specimen's 3D form
67
when a specimen is prepared for observation the object should always be viewed as a
wet mount
68
what stain was used for epithelial cells
methylene blue or iodine solution
69
epidermal cells cover the
surfaces of plant organs such as leaves. bulb of an onion is made of fleshy leaves.
70
stain used for onion cells
iodine (or methylene blue)
71
calculate length of onion cell
HPD/# of cells across
72
euglena is
a unicellular organism with a flagellum to facilitate movement
73
protoslo is
methyl cellulose solution
74
protoslo
slows the organism's swimming
75
euglena shows an
aversion to protoslo

Bio 110 Lab (5 decks)

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