BInary fission Flashcards
what is binary fission?
bacteria cell division
what are the 4 phases of bacterial growth in a growth curve?
- )lag phase
- )logarithmic phase
- )stationary phase
- )death phase
lag phase=?
period of adjustment
what are the characteristics of the lag phase?
a. )bacteria are adjusting to envitoment
b. )bacteria unable to adjust will NOT grow
c. )bacteria ARE metabolically active
d. )bacteria show NO signs of reproducing or dividing
what is the clinical application for the lag phase (AKA period of adjustment) ?
incubation period of disease (before the onset of symptoms)
logarithmic phase=?
period of bacteria GROWTH
what are the characteristics of the logarithmic phase?
a. )bacteria cells are actively dividing (divide at maximun rate)
b. )growth rate influenced by 2 things
c. )bacteria are at metabolic PEAK
d. )bacteria are MOST susceptible to antibiotics and antiseptics
what are the 2 thing that influence the growth rate?
- )physical/chemical enviroment
2. )genetic make up
what is the clinical application for the logarithmic phase (AKA period of bacterial growth)?
onset of symptoms, acute phase of disease sypmtoms
Stationary phase=?
maximum # of cells the environment can support
what are the characteristics of the stationary phase?
a. )NO increase in population size
b. )bacteria cell switch to LOWEST metabolic rate
c. )cells tend to be LESS susceptible to antibiotics
d. )spore formers from endospores as a protective mechanism
e. )if growth conditions become favorable again, cells could return to log phase of growth
what are the 2 factors that affect no increase of population size?
- )death rate= reproduction rate
2. )every cell in the population stops dividing but none die
what is the clinical application for stationary phase (AKA maximum # of cells the environment can support)?
symptoms starting to diminish, fever diminish
death phase=?
a dead cell is a cell that can no longer divide even if conditions improve
what the characteristics for death phase?
a. )cell death occurs ar an exponential rate
b. )death rate is at MAXIMUM rate
what is the clinical application for death phase (AKA a dead cell that can no longer divide even if conditions improve)?
recovery!
LOOK at growth curve at page 34 in your notes!
it WILL be on the exam my niggah
what is binary fission NOT?
mitosis or meiosis cell division processes.
what is the outline of binary fission process? (there are 6 steps)
- )Binary fission begins with 1 parent bacteria cell that contains a single chromosome loop of DNA.
- )In preparation for cell division, the parent cell duplicates or replicates its DNA chromosome.
- )This results in a Parent cell with 2 complete, identical copies of the original parent DNA chromosome. (DNA duplication is an essential step as the parent cell is preparing to divide into 2 daughter cells so that each new daughter cell will have a complete replica of the original parent DNA molecule.)
- )At the end of DNA replication stage, the parent bacteria cell will increase in size and eventually elongate or lengthen.
- )A cell wall will form in the center of the parent bacteria cell separating the 2 DNA molecules and equally dividing the cytoplasm and cell contents. This results in 2 daughter cells of equal size, each with a DNA molecule identical to the original parent cell.
- )At the end of Binary Fission, the 2 newly created daughter cells are clones of the original parent, identical genetically to the parent cell.
define generation time
time it takes a single cell or population to double in size
what is generation time influenced by?
genetic make up, environment conditions
do all microbes dehydrate at the same rate?
NO
do all microbes require water for growth?
YES
what are the top 3 elements are required for cell growth and metabolism?
carbon, hydrogen, & oxygen
what is the #1 element found in organic molecules?
carbon
what is the #1 element that all living require?
carbon
what is nitrogen important for?
production of proteins and nucleic acids including the DNA molecule
what is phosphorous important for?
production of phospholipids and ATP
what is sulfur important for?
certain protein molecules in the cell
photo=?
sunlight
troph=?
how a cell obtains its food
what source of energy does phototrophic bacteria use?
absorb sunlight or UV light to provide energy for the cell
can pathogenic bacteria be categorized as phototrophic phototroph?
NO
chemo=?
chemicals
what source of energy does chemotrophic bacteria use?
use organic or inorganic chemical molecules to provide energy for the cell
can pathogenic bacteria be categorized as chemotrophic chemotroph?
YES
where does phtotoautotroph get its energy source?
sunlight
where does phtotoautotroph get its carbon source?
carbon dioxide
define phtotoautotroph
bacteria that obtain their energy from the sun and the cells can make their own food, therefore, this bacteria is SELF SUFFICIENT
what is and example photoautotroph?
cyanobacteria
where does chemoautotroph get its energy source?
INORGANIC molecules like SULFUR or NITROGEN
where does chemoaurotroph get is carbon source?
carbon dioxde
what is an example of chemoaurotroph?
archeabacteria
can human pathogens be classified as chemoaurotroph?
NO
can human pathogens be classified as photoautotroph?
NO
auto=?
SELF
heterotroph=?
NOT self sufficient
where does photoheterotroph get it energy source?
sunlight
where does photoheterotroph get its carbon source?
ORGANIC molecules like protein or sugar
what is an exmaple of phtotoheterotroph?
purple non sulfur bacteria
can human pathogens be photoheterotroph?
NO
where does chemoheterotroph get its energy source?
ORGANIC molecules
where does chemoheterotroph get its carbon source?
ORGANIC molecules
what are examples of chemoheterotroph?
pathogen & saprobe
what is the temperature required for psychrophiles to live?
-5C, -20C COLD AF!!!!
what is the temperature required for mecophiles to live?
20C-50C temperate soil, human body
what is the temperature required for thermophiles to live?
50C-80C HOT
what is the temperature for extreme thermophiles to live?
> 80C HOT AF!!!!
define obligate aerobe
NEED oxygen to grow REPRODUCE
define obligate anerobe
KILLED in the presence of oxygen
define aerotolerant organisms
GROW in the presence or absence of oxygen but DO NOT use Oxygen and, therefore, grow BETTER in ANAEROBIC conditions.
define facultative anaerobe
GROW in the presence or absence of Oxygen, PREFER the presence of Oxygen and, therefore, grow BETTER in AEROBIC conditions
what conditions are required for microaerophile to grow?
LOW oxygen concentration 10%
HIGH carbon dioxide concentration 90%
what is the perfect environment to grow and study microaerophiles?
candle jar
0-7 ph =?
ACID
7-14 ph =?
BASE (alkaline)
7 ph=?
NEUTRAL
most bacteria prefer the __ ph
7
what is the ph range fore most bacterias?
6-8
saprobe=?
decomposer