BC - L5

Question 1

Topics?

Answer 1

passive immunity & HIV
modulating the immune system
new therapeutic uses for mAbs

Question 2

HIV subtopics?

Answer 2

completed vaxine trials
selection..
HIV entry process
enriching for Abs the recognise CD4 binding site
large scale isolation of bNt mAbs
arms race
improving HIV mAbs
types of therapy tested

Question 3

What happened with the completed HIV vaccine clinical trials?

Answer 3

no protection observed

Question 4

selection?

Answer 4

HIV has a very large number of genetic variants circulating at the same time (compared to flu) –> Abs dont function
only bNt mAbs

Question 5

Describe the HIV entry process

Answer 5

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Question 6

Abs for CD4?

Answer 6

delRSC3 is a mimic of virus that binds to the CD4 receptor

Question 7

How to isolate bNt mAbs fro memory cells?

Answer 7

sort single IgG B Cells that bind a modified gp160 trimer called 2CC
recover the IgV genes from each cell
re-express as a recombinant, complete IgG in a test tube

Question 8

What is the arms race, descirbe

Answer 8

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Question 9

Example of improving mAbs for HIV

Answer 9

G –> aromatic

Question 10

Types of therapies?

Answer 10

mono - ineffective
tri - partly effective
penta - effective

Question 11

Modulating the immune system?

Answer 11

Ipilimumab
T cell activation
activation of CTLA4 & PD-1 pathways in immune response

Question 12

New therapeutic uses for mAbs?

Answer 12

CAR

anti-CD19 CAR T cell therapy

Question 13

Describe the CAR pathway

Answer 13

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