Brainscape's Knowledge GenomeTM


Top Flashcards (Certified)
All Flashcards

Medical Microbiology Labs > Bacteroides spp > Flashcards

Bacteroides spp Flashcards

1
Q

Bacteroides culture conditions

A

Anaerobic 37C blood agar

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

Anaerobic bacteria

A

Clostridioides
Bacteroides

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

Anaerobic Gram negative bacillus

A

Bacteroides

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

Bacteroides colonial morphology

A

1-3mm, round, shiny grey, no haemolysis

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

Gram stain

A

Gram negative bacilli
Small slender rods
clusters
sometimes coccobacilli

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

Biochemical tests

A

KOH positive
Catalase positive
Oxidase Negative

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

Presumptive Bacteroides spp tests

A
  1. Anaerobic growth
  2. Metronidazole susceptibility test
  3. Anaerobic identification discs - MID8
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

Anaerobic growth + metronidazole susceptibility test procedure

A
  1. Lawn inoculate blood agar purity plate with test organism + PC ( P. aeruginosa) + NC (B. fragilis) and incubate AEROBICALLY overnight at 37C
  2. Lawn inoculate blood agar purity plate with test + controls (PC = B. fragilis, NC = P. aeruginosa).
  3. Place metronidazole disc on secondary inoculum of test organism.
  4. Incubate ANAEROBICALLY for 48hrs using anaerobic jar and AnaeroGen sachet.
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

Anaerobic growth + metronidazole susceptibility test results

A

Anaerobic organisms only grow anaerobically (no O2) AND anaerobic organisms are SUSCEPTIBLE to metronidazole = produce zone on inhibition of growth around disk.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

Anaerobic identification discs - MID8 test procedure

A
  1. Suspend test organism in 5ml water = dense suspension
  2. Lawn suspension on BA plate
  3. Using forceps place MID9 mastring on surface of BA plate.
  4. Incubate anaerobically 37C for 48hrs.
  5. Measure inhibition zones using digitial calipers.
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

B fragilis MID8 sensitivity results

A

E + RP sensitive
Resistant to the other 4 (CO, PG, K, VA)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

MID8 code and corresponding drug
E
RP
CO
PG
K
VA

A

E = erythromycin
RP = rifampcin
CO = colistin sulphate
PG = penicillin G
K = kanamycin
VA = vancomycin

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

Additional confirmatory tests

A

Vitek, MALDI-TOF

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

Bacteroides infections

A

Normal commensal of human colon so it is an endogenous infection which occurs when they gain access to tissue/blood following bowel perforation - surgery/trauma.
Causes ABSCESSES - abdominal or peritoneal

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

Bacteroides treatment

A
  1. AST - AMR is common
  2. Metronidazole.
How well did you know this?
1
Not at all
2
3
4
5
Perfectly

Medical Microbiology Labs (14 decks)

Brainscape helps you reach your goals faster, through stronger study habits.
© 2025 Bold Learning Solutions. Terms and Conditions