bacterial structure & growths Flashcards
Similarities between bacteria and eukaryotes
biochem units amino acids nucleotides fatty acids vitamins energy metabolism biosynthesis
Difference between bacteria and eukaryotes
Cell wall
70S ribosome
No ER/mitochondria
No mitotic spindles
Porins are on ___
Gram negative
Periplasmic space are in ___
Gram negative
Outer membrane are on ____
Gram negative
Pili on ___
both
lipopolysaccharides (LPS) are on ___
Gram negative
Teichoic acids are on ___
Gram positive
Lipoteichoic acids are on ___ function to ____
Gram positive, anchor cell wall to membrane
Cytoskeleton for bacterial (3) (what, similar eukaryotic, function)
Ftz - tubulin - division
MreB - actin - shape/polarity/chrom separation
CreS - intermediate filaments - shape
LPS located on?
gram neg, outter leaflet of outer membrane
LPS parts
Lipid A - endotoxins
Core polysaccharides
O side oligosacchardies (somatic antigens = O antigens)
Energy sources (2)
ATP
Electron transport system (proton motive force)
Significance of nucleoid (vs nucleus)
Transcrption and translation happens simultaneously
Bacterial growth curve: Lag phase
Inoculum - adjustment to new evironment - proper intracellular environment to optimal grow
Bacterial growth curve: Exponential phase
Rate proportional to amount of bacteria
Doubling/generation time
Bacterial growth curve: Stationary phase
Grow = death; nutrition vs toxin
Bacterial growth curve: Death phase
decline - spontaneous cell lysis/autolysis
Aerobes characteristics
need O2, cannot ferment
Anaerobes characteristics
Kill by O2, only ferments
Indifferent
aerobic tolerant - will ferment
Facultative
respire with O2, ferment with O2
Microerophilic
best with low O2
Fermentation vs respiration
Ferm: organic compound - e- donnor & acceptor - no net oxidation
Resp: electron transport system
- Use O2 or amino acids/nitrate/nitrite as final e acceptors
sporulation
turns to spores - dehydrated, resistant, no metabolic activity
Germination
spore –> vegatative state when conditions improves
Antimicrobials targets:
cell wall outer/cytoplasmic membrane ribosomes nucleic acid syn metabolic inhib