Bacterial Structure and Growth

Question 1

Main diff of euk and prok

Answer 1

Prokaryotes - no membrane bound organlles…70S ribosomes…binary fission…singular chromosome…no cytoskeleton and chemically ocmplex cell wall

Question 2

Gram negative

Answer 2

LPS

Lipid A is portion of LPS that is main antigenic portion

Question 3

Gram positive

Answer 3

Thick peptidoglycan layer with lipoteichoic acid and teichoic acid

Question 4

Gram positive rod that is anaerobe

Answer 4

Clostridium

Question 5

Gram positive rods

Answer 5

Listeria, bacillus, clostridium

Question 6

Gram positive cocci catalase positive

Answer 6

Staph (clusters)

Question 7

Gram positive cocci, catalase negative

Answer 7

Strep (chains)

Question 8

Gram positive cocci, catalase positive, coagulase positive and negative

Answer 8

Positive - staph aureus

Negative - staph epidermidis

Question 9

Gram positive cocci catalase negative hemolytic alpha/beta, gamma

Answer 9

Strep pneumoniae - alpha
Strep pyogenes, agalctiae - beta
Enterococcus - gamma

Question 10

Gram positive cocci catalase negative hemolytic beta group A and B

Answer 10

Group A - strep pyogenes - bacitracin sensitive

Group B - strep agalctiae - bacitracin resistant

Question 11

Gram negative cocci

Answer 11

N meningitidis and gonorrhoeae

Question 12

Gram negative cocci maltose fermenter/non fermenter

Answer 12

Fermenter - meningitidis

Non - gonorrhoeae

Question 13

Gram negative coccoid

Answer 13

Heomophilus infuenzae (needs factors 5 and 10)
Bordetella pertussis

Question 14

Gram negative rods lactose fermenters and non fermenters

Answer 14

Fermenters - klebsiella and E coli

Non - Shigella, salmonella, proteus, pseudomonoas

Question 15

Gram negative rods non lactose fermenter oxidase negative and positive

Answer 15

negative - shigella, salmonella, proteus

Positive - pseudomonas

Question 16

Bacterial capsules are

Answer 16

Usually polysaccharide

Question 17

Spirochetes

Answer 17

Helical shape, corkscrew

Treponema is example

Question 18

Obligate intracellular pathogens

Answer 18

Chlamydia and rickettsia

Question 19

No cell wall

Answer 19

Mycoplasma

Question 20

Mycobacteria

Answer 20

Cell wall of mycolic acid and lipoarabinomannan (need acid fast stain)

Question 21

Other name of acid fast stain and what is quality of mycolic acid

Answer 21

Ziehl- Neelsen stain

Very waxy

Question 22

Lag phase

Answer 22

Acquiring nutrients and preparing for growht

Question 23

Exponential phase

Answer 23

Actively dividing

Question 24

Stationary phase

Answer 24

Point at whih bacteria are using up nutrients present as fast they can be provided

Question 25

Death phase

Answer 25

Too many bacteria for the environment

Question 26

Selective media

Answer 26

Inhibits growht of a certain type

Question 27

Differential media

Answer 27

Supports growht of many bacteria and includes indicator

Question 28

Differential media of blood agar

Answer 28

Alpha - green is partial hemolysis
Beta - completely lyse and yellow
Gamma - clear and no hemolysis