Bacterial Growth and Metabolism

Question 1

Aerobic respiration

Answer 1

Glucose converted to Co2 and H2O forms ATP via substrate level and oxidative phosphorylation

Question 2

Anaerobic respiration

Answer 2

Like aerobic but O2 is not final e acceptor

Question 3

Fermentation

Answer 3

Absence of O2…and following substrate level phosphoryklation, bacteria ferment pyruvic acid (fewer ATP)

Question 4

Obligate aerobs

Answer 4

Only aerobic growth
Towards top near high oxygen
Presence of catalase adn superoxide dimutase allows oxygen to be neutralized

Question 5

Facultative anaerobes

Answer 5

Both aerobic and anaerobic
Growht is best where oxygen is but can also grow in other areas
Still has catalase and SOD

Question 6

Obligate anaerobes

Answer 6

Only anaerobic growht
Only grow where no oxygen
Lacks enzymes to neutralize harmful oxygen forms

Question 7

Microaerophiles

Answer 7

Only aerobic growht but only at low concentration
Grow where low concentration of oxygen has diffused into media
Produce lethal amounts of toxic oxygen if exposed to atmospheric oxygen

Question 8

Tb vs. E coli growht

Answer 8

Tb - almost 24 hours

E coli - 20-30 minutes

Question 9

Bacterial exponential growht

Answer 9

Cell numbers increase by factor of 2 each division because of binary fission

Question 10

Exponential phase

Answer 10

Bacteria actively transport nutrients and synthesize all cell components
Maximally vulnerable to antimicrobials

Question 11

Stationary phase

Answer 11

Bacteria minimize active trasnport, cease divisoin, and limit biosynthesis to that required for cell integrity
Most antimicrobials are ineffective

Question 12

Why do you need to take entire course of antibiiotics?

Answer 12

Will kill bacteria in the growth phase but NOT in the stationary phase if you don;t finish…bacteria re-initiate growht