Bacterial Regulation Flashcards
2 approaches to regulation
gene regulation, enzyme/protein regulation
Other name for house keeping genes
Constitutive genes
Proteins synthesized when needed
Inducible genes, (such as catabolic genes)
Proteins repressed when not needed
Repressible genes (such as anabolic genes)
Catabolic genes
Inducible
Anabolic Genes
Repressable
Lac Z
Beta-galactosidase,
Hydrolyzes monomers
Lac Y
Lactose permease
Lac A
Acetylates galactosides with Acetyl-Coa
Lac operon repressor gene
LacL (naturally active)
CAP
Catabolite activator protein
CAP regulatory system
Cyclic AMP activated by PEP system, when no glucose present
mRNA attenuation
production of a shortened mRNA, terminated within the leader sequence
Negative control of the TRP operon
TRP repressor, tryptophan acts as a corepressor
Antiterminator stem loop in trp mRNA
2-3 stem loop
riboswitches
cis element that forms a loop in the mRNA to block access to the shine-delgarno sequence
Regulon
genes or operons controlled by single regulatory protein
Modulon
operon network under control of common protein (CAP system)
Phosphorelay system
turn many genes on or off in response to environmental conditions
2 parts of a Phosphorelay system
sensor kinase and response regulator (DNA binding protein)
Env Z
sensor kinase for osmolarity
Quorum sensing molecule for Lux operon
N-actyl-homoserine-lactone (AHL) binds to LuxR (Lux Activator)
Methyl accepting chemotaxis proteins regulate what?
Flagellar motion (direction)
Which protein interacts with flagella
CheY
