Bacterial Growth and Multiplication Flashcards

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1
Q

What are bacteria with simple needs?

A

Glucose/phosphate/sulphate/ammonia e.g. E. coli

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2
Q

What are fastidious bacteria?

A

Have complex nutritional needs i.e. aa, fatty acids, vitamins, nucleotides (e.g. Haemophilus)

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3
Q

What are usually the bacteria with unknown needs?

A

Intracellular parasites - unethical to grow inside host to be able to test (e.g. Rickettsia)

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4
Q

What are the 3 groups of bacteria defined by nutritional requirements?

A

Simple, fastidious, unknown

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5
Q

What are obligate aerobes?

A

Need oxygen to survive

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6
Q

What are microaerophilic bacteria?

A

Require reduced amount of oxygen (5%ish) - usually intestinal

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7
Q

What are facultatively anaerobic bacteria?

A

Can grow without oxygen but do not have to (intestinal or wounded tissue)

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8
Q

What are strictly anaerobic bacteria?

A

Can only multiply in absence of air

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9
Q

What are the byproducts of aerobic metabolism?

A

Hydrogen peroxide

Superoxide radical

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10
Q

How are aerobic bacteria able to deal with the byproducts of aerobic metabolism?

A
Catalase (deals with H2O2) 
Superoxide dismutase (deals with superoxide radicals)
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11
Q

What are the barriers to nutrient uptake?

A

Outer membrane and plasma membrane

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12
Q

What are the 3 mechanisms of nutrient uptake?

A

Passive diffusion, facilitated diffusion (permeases), active transport

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13
Q

What are the basic byproducts of fermentation?

A

Acids, CO2, H2, alcohols

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14
Q

What type of bacteria does lactic acid fermentation utilise?

A

Streptococcus

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15
Q

What type of bacteria does ethanol fermentation utilise?

A

Yeasts

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16
Q

What type of bacteria does mixed acid fermentation utilise?

A

E. coli

17
Q

What are the byproducts of respiration?

A

CO2, ATP, NADH2, NADPH2

18
Q

What happens to the NADH/NADH2 of respiration?

A

Enters the electron transport chain to produce ATP (via proton motive force)

19
Q

What is the terminal electron acceptor in aerobic respiration?

A

Oxygen (makes water)

20
Q

What are the terminal electron acceptors in anaerobic respiration?

A

Nitrate, sulphate, carbon dioxide (methane)

21
Q

What are the 4 different types growing of media?

A

Nutritionally simple
Enriched (non-specific)
Selective
Differential

22
Q

What is selective media?

A

Inhibition/suppression of other bacteria OR specific enrichment of target bacteria

23
Q

What is differential media?

A

Provides visual differentiation of bacterial colonies

24
Q

What is the lag phase?

A

Readjustment period

25
Q

What is the log phase?

A

Exponential growth

26
Q

What is the doubling time of E. coli?

A

20 minutes