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VMCB 121 > Bacterial Growth > Flashcards

Bacterial Growth Flashcards

1
Q

Orderly increase of all the chemical constituents

A

Growth

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2
Q

The increase in the number of microbial cells in a population.

A

Bacterial growth

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3
Q

The change in cell number or cell mass per unit tie.

A

Growth rate

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4
Q

Pattern of population increase

A

Exponential growth

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5
Q

Th time interval for the formation of two daughter cell from one.

A

Generation time

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6
Q

How do bacteria reproduce?

A
  1. Binary fission
  2. Budding (Asexual reprodution)
  3. Fragmentation
  4. Spore formation
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7
Q

Phases of bacterial growth curve?

A
  1. Lag phase
  2. Log phase
  3. Stationary phase
  4. Death phase or decline phase
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8
Q

It is the time period in which bacteria is metabolically active but do not divide is called?

A

Lag phase

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9
Q

During this phase bacteria divides continuously at constant rate and the number of bacteria increase exponentially.

A

Log phase

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10
Q

The bacteria reaches a state during which there is no net increase in bacterial population

A

Stationary phase

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11
Q

In this phase, the number of bacteria decrease continuously exponentially.

A

Death phase or decline phase

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12
Q

Bacteria have the maximum cell size towards the end of the lag phase.

A

Lag phase

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13
Q

Cells are smaller and stain uniformly in the log phase

A

Log phase

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14
Q
  • Cells frequently are Gram variable and show irregular staining due to the presence of intracellular storage granules.
  • Sporulation also occurs at this stage
A

Stationary phase

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15
Q

Involution forms are common

A

Decline phase

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16
Q

The time interval required for the cells (or population) to divide.

A

Generation time

17
Q

Time interval in hours or minutes?

A

t

18
Q

Number of bacteria at the beginning of a time interval

A

B

19
Q

Number of bacteria at the end of the time interval?

A

b

20
Q

Number of generations (number of times the cell population doubles during the time interval)

A

n

21
Q

Equation is an expression of growth by binary fission

A

Bx2n

22
Q

A measured volume of a bacterial suspension is placed within a defined area on a microscope slide.

A

Direct Microscopic Count

23
Q

For longer eukaryotic microorganisms

A

Hemocytometer

24
Q

For bacteria?

A

Petroff-Hausser Counting Chamber

25
Q

Electronic counting chambers count numbers and measured size distribution of cells.

A

Electronic count

26
Q

It gives accurate results with larger cells; not useful in counting bacteria

A

Coulter counter

27
Q

It measures the number of living cells, cells capable of multiplication.

A

Viable counts

28
Q

Involve plating out (spreading) a sample of a culture on a nutrient agar surface.

A

Plating Techniques

29
Q

Each colony that can be counted in Plating Technique is called?

A

Colony Forming Unit (CFU)

30
Q

This employ a variety of instruments to determine the amount of light scattered by a suspension of cells.

A

Turbidity Measurements

31
Q

More rapid; sensitive technique; measures absorbance units

A

Spectrophotometry

32
Q
  • For cells growing in liquid samples
  • Useful technique for measuring the growth of fungi
A

Microbial Dry Weight Determination

33
Q

The total quantity of the cell constituent is directly related to the total microbial cell mass

A

Determination/Analysis of Cell Constituent

34
Q
  • A microbial culture that contains cells that are in the same growth stage
  • Extensively used to address questions regarding cell cycle and growth
A

Synchronous culture of bacteria

35
Q

A hypothetical situation in which the number of cells in a culture would increase in a stair-step pattern, dividing together at the same time.

A

Synchronous growth

36
Q

A natural situation in which an actual culture has cell dividing at one rate and other cells dividing at a lightly slower rate.

A

Nonsynchronous growth

37
Q
A

VMCB 121 (6 decks)

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