B1 microscopy Flashcards
What is the primary function of a microscope?
To magnify small objects so they can be seen in detail.
True or False: A light microscope uses visible light to illuminate specimens.
True
Fill in the blank: The part of the microscope that holds the objective lenses is called the _______.
nosepiece
What is the maximum magnification typically achievable with a light microscope?
Around 1000x
What is the difference between resolution and magnification?
Resolution is the ability to distinguish two points as separate, while magnification is the enlargement of an image.
Which part of the microscope adjusts the amount of light that reaches the slide?
Iris diaphragm
What type of microscope uses electrons to achieve high magnification?
Electron microscope
True or False: The higher the magnification, the lower the resolution.
False
Fill in the blank: The _______ lens is the lens closest to the specimen.
objective
What is the purpose of the stage on a microscope?
To hold the slide in place for viewing.
Which type of microscopy allows for viewing live cells?
Phase contrast microscopy
What is the unit of measurement commonly used to describe the size of cells?
Micrometers (µm)
True or False: A cover slip is used to protect the objective lens and flatten the specimen.
True
What is the function of the ocular lens?
To magnify the image further for the viewer’s eye.
Fill in the blank: In microscopy, the term _______ refers to the clarity of the image.
resolution
Which part of the microscope can be adjusted to focus the image?
Coarse and fine focus knobs
What type of specimen preparation involves staining to enhance visibility under a microscope?
Histological preparation
Fill in the blank: The _______ is the light source in a light microscope.
lamp
What is the main advantage of using an electron microscope?
It provides much higher magnification and resolution than light microscopes.
True or False: The total magnification is calculated by multiplying the magnification of the ocular lens by the magnification of the objective lens.
True
What is the main limitation of light microscopes?
They cannot resolve structures smaller than about 200 nanometers.
Fill in the blank: The term _______ refers to the ability of a microscope to distinguish between two points.
resolution
What is the function of the fine focus knob?
To make small adjustments to the focus of the image.
Which type of lenses are used in a light microscope?
Convex lenses
What is the primary use of a scanning electron microscope (SEM)?
To create detailed three-dimensional images of surfaces.
Fill in the blank: A _______ microscope uses a beam of electrons instead of light.
transmission
