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🦠RT > Autres > Flashcards

Autres Flashcards

1
Q

Hazard diamond

  • bleu area=
  • red area=
  • yellow area=
  • white area=
A
  • bleu area= health
  • red area= flammabilty
  • yellow area= reactivity
  • white area= special, W=unsual reactivty with water, OX=oxidizer
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2
Q

Class for fire, material

  • A
  • B
  • C
  • D
A
  • A : Cloth, wood, paper
  • B : liquids
  • C : electrical
  • D : metals
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3
Q

Class for fire, type extinguisher

  • A
  • B
  • C
  • D
A
  • A : A(water) or ABC (dry chemical)
  • B : ABC or BC(co2)
  • C : ABC or BC
  • D : firefighters
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4
Q

EDTA

  • Color stopper
  • action
  • uses
  • comments
A
  • Color stopper: lavender
  • action: stop clot by chelating calcium
  • uses: CBC, diff, sed rate
  • comments: at least half full
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5
Q

Heparin

  • Color stopper
  • action
  • uses
  • comments
A
  • Color stopper: green
  • action: stop clot by neutralizing thrombin
  • uses: osmotic fragility, plasma hgb, blood gases
  • comments:best to avoid hemolysis
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6
Q

Citrate de sodium

  • Color stopper
  • action
  • uses
  • comments
A
  • Color stopper: light blue
  • action: stop clot by binding calcium
  • uses: coagulation
  • comments: 9:1 blood:anticoag ratio, reduce anticoag if Hct is more then 55%, if using a butterfly use a discard tube
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7
Q

Fluoride de sodium

  • Color stopper
  • action
  • uses
  • comments
A
  • Color stopper: gray
  • action: not anticoag inhibits glycolysis
  • uses: glucose, lactic acid and alcohol
  • comments: add k oxalate for anticoag
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8
Q

Which color of tube we need for serum?

A

Red or gold

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9
Q

difference between plasma and serum

A

plasma contains fibrinogen

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10
Q

Where do we draw blood if presence of

  • Mastectomy
  • hematoma
  • IV
A
  • Mastectomy : other arm
  • hematoma : under it
  • IV : opposite arm or finger pic, otherwise turn off IV 2 min
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11
Q

Which test tube need to be chilled?

A

Ammonia and lactic acid (slurry)

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12
Q

Which test need tube to be warmed?

A

cold agglutinins and cryoglobulins (37oC)

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13
Q

Which test need tube to be protected by the light?

A

bilirubine (use aluminum foil)

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14
Q

What does fasting mean?

A

nothing to eat or drink(except water) for at least 8 hr

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15
Q

Spin tubes within how much time after collecting? and speed/time

A

2 hours, 1,000-1,300 RCF for 10-15min (except citrate tube)

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16
Q

how to achieve PPP platelet poor plasma

A

1500 RCF for 15min (for citrate tubes)

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17
Q

How do we freeze whole blood

A

you cant freeze whole blood (must seperate de plasma and culot)

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18
Q

What type of glass we use to reduced light exposure

A

low actinic or amber/red glass

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19
Q

What type of glass we use to alkaline solutions? (best)

A

boron free

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20
Q

What type of glass is most common and can be used for autoclave/heat resistance?

A

borosilicate (kimax and pyrex)

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21
Q

What type of glass is diposible(least expensive) but not very temperature changing resistant?

A

flint glass (soda-lime color)

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22
Q

What type of glass we use to for Heat, chemical and ELECTRICAL tolerance?

A

High silica

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23
Q

What type of glass we use to resist clouding due to alkali and scratching?

A

Aluminosilicate (corex)

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24
Q

Volumetric pipet how to use

A

touch off last drop against wall

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25
Q

Ostwald-Folin pipet how to use

A

for viscous fluids, ertched(frost) ring means blowout

26
Q

seroligical pipet how to use

A

not accurate use for serial dilutions and mesuring reagents, ertched(frost) ring means blowout

27
Q

Mohr pipet how to use

A

no blowout

28
Q

Micropipet how to use

A

TC = to contain, 1-1,000uL

29
Q

Most pure graded chemical is

A

analytic reagent grade (meets specification american chemical society)

30
Q

Type I water =
Type II water=
Type III water=

A

Type I water = ultrapure
Type II water= pure (can make buffer)
Type III water= primary grade water

31
Q

What does the condenser does on a microscope?

A

focuses the light on specimen

32
Q

What does the ocular does on a microscope?

A

eye piece, 10x

33
Q

What does the objectives does on a microscope?

A

low power 10x, high power 40x and immersion oil 50-100x

34
Q

type of bulb for brightfield microscope?

A

tungsten

35
Q

Kind of microscope for ID treponema pallidum

A

dark field (black background)

36
Q

Kind of microscope for fluorescent antibody stains

A

fluorescent

37
Q

Kind of microscope for platelet count or urine sediments?

A

phase contrast (has phase condenser and objectives)

38
Q

Kind of microscope for ID urine crystals, synovial fluid and fat oval bodies

A

Polarizing (has a polorizing filter below condenser), objects reflect light birefringent

39
Q

What is quality assurance?

A

process by which the lab ensures quality results by closely monitoring preanalytical, analytical and postanalytical stage of testing

40
Q

Mean

A

racine carée (somme(x-moyenne)^2 / n-1)

41
Q

Standard deviation

A

SD =racine carée (somme(mean-x)^2 /n-1)

x= each value
n= number of value
42
Q

Coefficient of variation, formula

A

CV% = (SD / mean) x100

43
Q

Coefficient of variation, high the precision _ the CV

A

lower the CV

44
Q

% of values fall within +/- 1SD

A

68%

45
Q

% of values fall within +/- 2SD

A

95%

46
Q

% of values fall within +/- 3SD

A

99%

47
Q

Coefficient of variation should be under %

A

5%

48
Q

what does normal distribution mean?

A

x(average)=md(median)=mo(mode)

49
Q

Variance formula

A

S^2

50
Q

limite controle acceptable formula

A

x +/- 2s (average +/- 2xmean)

51
Q 
Westguard rules
1 2s = 
1 3s = 
2 2s = 
R 4s=
4 1s = 
10x =
A

1 2s = alarm, 1 control exceeds +/- 2s
1 3s = reject, 1 control exceeds +/- 3s
2 2s = reject, 2 control consecutive exceeds +/- 2s
R 4s= reject, 2 control exceeds +/- 4s
4 1s = reject, 4 control exceeds +/- 1s
10x = reject, 10 control consecutive same side of average

52
Q 
Micro =
milli=
nano=
pico=
fento=
A 
Micro =10^-6
milli=10^-3
nano=10^-9
pico=10^-12
fento=10^-15
53
Q

Dilution formula

A

vol specimen / vol specimen + vol diluent

54
Q

how do you prepare 1:10 dilution of urine?

A

1 part urine and 9 part diluent

55
Q

How many ml of 95% alcohol are neede to prepare 100mL of 70% alcohol?

A

C1V1=C2V2
(95)(v)=(70)(100)
v= 73.7mL

56
Q

What is the concentration in % of a solution that contains 8.5g of NaCl per L?

A

8.5g/1000ml x X/100ml
1000X=8.5 x 100
c=0.85%

57
Q

A manual glucose assay gave the following results : abs of 100mg/dL , standard=0.3. Abs of patient =0.4. what is the concentration of glucose of the patient?

A

Use beer law

  1. concentration pt = abs unknown / abs standard x concentration standard
  2. concentration patient = 0.4/0.3 x 100
  3. concentration patient = 133mg/dL
58
Q

What is the molarity of a solution that contains 45g of NaCl per L? (atomic weight, Na=23 et Cl=35.5)

A 
M= (g/L) / (gram molecular weight)
M= 45/58.5
M= 0.77
59
Q

Formula for pH calculation

A

-log(H)

60
Q

temperature PCR

A

95oC, 55oC et 72oC

🦠RT (11 decks)

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