Automation of Machines Flashcards

1
Q

Photons travelling in waves

A

Electromagnetic radiation

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2
Q

Distance between two peaks

A

Wavelength

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3
Q

Distance between peak and trough

A

Amplitude

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4
Q

Falls in between 400 nm to 700 nm

A

Visible lights

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5
Q

The concentration of a substance is directly proportional to the amount light absorbed or inversely proportional to the logarithm of the transmitted light

A

Beer lawbert law (Beers law)

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6
Q

Method of determining the concentration of substance in solutions; measuring amount of light absorbed by that solution

A

Spectrophotometer

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7
Q

Most common light source in visible and near infrared regions

A

Incandescent tungsten or tungsten iodide lamp

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8
Q

Most common used for UV work

A

Discharge lamp and mercury arc lamp

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9
Q

Will select your desired wavelength

A

Monochromators

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10
Q

Most common used in monochromators

A

Diffraction grating

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11
Q

Round vs square cuvette

A

Preferred square cuvette

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12
Q

Used for application in the visible range or infrared

A

Glass

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13
Q

For application requiring UV radiation

A

Quartz

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14
Q

Layer cell or photo cell; least expensive

A

Barrier

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15
Q

Used in instrument designed to be extremely sensitive to very low light level and light flash

A

Photomultiplier tube

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16
Q

Measures the quantity of light reflected by a liquid sample that has been dispensed onto a grainy or fibrous solid support

A

Reflectometry

17
Q

Measurement of concentration is done by detecting absorption electromagnetic by atom rather than molecules

A

Atomic absorption spectrophotometry

18
Q

Usual light source of AAS

A

Hallow cathode lamp

19
Q

Break chemical bond and form free unexcited atoms serves as sample cells (AAS)

A

Flame

20
Q

Used to isolate the desired wavelength (AAS)

A

Monochromators

21
Q

Measurement of light emitted by excited atoms

A

Flame Photometry

22
Q

Measurement of the concentration of the solutions that contain flourescing molecules; used to measure small particles

A

Flurometry

23
Q

Used for protein blotting, immunoassay, pharmacological and toxicological testing

A

Chemiluminescence

24
Q

Measurements made with spectrophotometer to determine a concentration of particulate matter in sample

A

Turbidimetry

25
Q

Light scattered by a small particles is measured at an angle to the beam incident to the cuvette

A

Nephelometry

26
Q

Measure particles which are too larger for spectrophotometry

A

Nephelometers

27
Q

Measurement of the current or voltage generated by the activity of spiecific ion

A

Electrochemistry

28
Q

Measurement of potential voltage between two electrode in a solute to measure analyte concentration

A

Potentiometry

29
Q

Measurement of the current flow produced by an oxidation reduction reaction

A

Amperometry

30
Q

Electrochemical titration in which titrant is electrochemically generated

A

Coulometry

31
Q

Potential is applied to an electrochemical cell and the resulting current is measured

A

Voltammetry

32
Q

Separation of charged compounds based on their electric charge

A

Electrophoresis