Automation of Machines Flashcards
Photons travelling in waves
Electromagnetic radiation
Distance between two peaks
Wavelength
Distance between peak and trough
Amplitude
Falls in between 400 nm to 700 nm
Visible lights
The concentration of a substance is directly proportional to the amount light absorbed or inversely proportional to the logarithm of the transmitted light
Beer lawbert law (Beers law)
Method of determining the concentration of substance in solutions; measuring amount of light absorbed by that solution
Spectrophotometer
Most common light source in visible and near infrared regions
Incandescent tungsten or tungsten iodide lamp
Most common used for UV work
Discharge lamp and mercury arc lamp
Will select your desired wavelength
Monochromators
Most common used in monochromators
Diffraction grating
Round vs square cuvette
Preferred square cuvette
Used for application in the visible range or infrared
Glass
For application requiring UV radiation
Quartz
Layer cell or photo cell; least expensive
Barrier
Used in instrument designed to be extremely sensitive to very low light level and light flash
Photomultiplier tube
Measures the quantity of light reflected by a liquid sample that has been dispensed onto a grainy or fibrous solid support
Reflectometry
Measurement of concentration is done by detecting absorption electromagnetic by atom rather than molecules
Atomic absorption spectrophotometry
Usual light source of AAS
Hallow cathode lamp
Break chemical bond and form free unexcited atoms serves as sample cells (AAS)
Flame
Used to isolate the desired wavelength (AAS)
Monochromators
Measurement of light emitted by excited atoms
Flame Photometry
Measurement of the concentration of the solutions that contain flourescing molecules; used to measure small particles
Flurometry
Used for protein blotting, immunoassay, pharmacological and toxicological testing
Chemiluminescence
Measurements made with spectrophotometer to determine a concentration of particulate matter in sample
Turbidimetry
Light scattered by a small particles is measured at an angle to the beam incident to the cuvette
Nephelometry
Measure particles which are too larger for spectrophotometry
Nephelometers
Measurement of the current or voltage generated by the activity of spiecific ion
Electrochemistry
Measurement of potential voltage between two electrode in a solute to measure analyte concentration
Potentiometry
Measurement of the current flow produced by an oxidation reduction reaction
Amperometry
Electrochemical titration in which titrant is electrochemically generated
Coulometry
Potential is applied to an electrochemical cell and the resulting current is measured
Voltammetry
Separation of charged compounds based on their electric charge
Electrophoresis