AutoImmune Disease II Flashcards
Anti-native DNA (nDNA) test:
- describe the test antigen
Crithidia luciliae, a flagellated parasite with kinetoplast that is rich in nDNA
Anti-native DNA (nDNA) test:
- Indicate which antibodies are detected
detects antibodies against double stranded DNA
Anti-native DNA (nDNA) test:
- appearance of a positive
Fluorescence of the kintoplast is a positive result (dot farthest away from flagella)
Anti-native DNA (nDNA) test:
- appearance of a negative
No fluorescence of kinetoplast (negative for anti nDNA)
Anti-native DNA (nDNA) test:
- Explain how test is used diagnostically
Positive result does not confirm SLE but nDNA antibodies are often found in SLE patients
Anti-native DNA (nDNA) test:
- What staining pattern should Anti nDNA antibodies produce
Either homogeneous or peripheral staining
Antibodies Against Extractable Nuclear Antigens (ENA)
- List antibodies that are detected
Smith, RNP, SS-A, SS-B, Jo-, Scl-70, and others (centromere B, chromatin, ribosomal P)
Antibodies Against Extractable Nuclear Antigens (ENA)
- antigen used in detecting antibodies
Same antigen as nDNA test: Crithidia luciliae ???
Antibodies Against Extractable Nuclear Antigens (ENA)
- Expected pattern in the ANA and the disease association for Smith
Speckled
SLE
Antibodies Against Extractable Nuclear Antigens (ENA)
- Expected pattern in the ANA and the disease association for RNP
Specked
SLE
Antibodies Against Extractable Nuclear Antigens (ENA)
- Expected pattern in the ANA and the disease association for SS-A
Speckled
Sjogrens
Antibodies Against Extractable Nuclear Antigens (ENA)
- Expected pattern in the ANA and the disease association for SS-B
Specked
Sjogrens
Antibodies Against Extractable Nuclear Antigens (ENA)
- Expected pattern in the ANA and the disease association for Scl-70
Homogeneous or Nucleolar
Scleroderma (PSS)
Antibodies Against Extractable Nuclear Antigens (ENA)
- Expected pattern in the ANA and the disease association for Jo-1
Cytoplasmic
Polymyositis
At IUHPL Serology Lab ENA Antibodies are detected by what? Principle?
Bioplex Multiplex Bead System
- patients antibodies bind to corresponding beads
- Labeled anti-IgG binds to antibody-coated beads
- analyzed by laser interrogation by the instrument
Advantages and Disadvantages of using only an outdated system for ANA testing
Advantage: efficient, little expertise
Disadvantage: only Ab’s against Ag’s used in test system will be detected. Others will be missed
How does the staining patterns of AMA and ASMA differ from those produced by by ANA’s in the ANA test
These produce cytoplasmic fluorescence in the ANA test
What autoimmune diseases are characterized by presence of AMA and ASMA antibodies?
Liver Problems: primary biliary cirrhosis, chronic active hepatitis and others
How are assays for AMA performed?
Indirect immunofluorescnce where rabbit kidney cells are the antigen
How are assays for ASMA performed?
Indirect immunofluorescnce where tissue sections of rat stomach are the antigen
Anti-cardiolipin antibodies
- what conditions are these antibodies found
- autoimmune disorders
- infections
- neoplastic conditions
- drug-induced lupus syndromes
- often associated with recurrent episodes of arterial or venous thrombosis and recurrent miscarriages
Anti-cardiolipin antibodies
- define antiphospholipid antibodies
A heterogenous group detected in a variety of patients with a variety of disorders
Anti-cardiolipin antibodies
- How are they indicated in the lab
Individual tests are done and clotting test for “lupus anticoagulant” are done
Anti-cardiolipin antibodies
- what is the effect of these on the non-treponemal tests for syphilis
They are “accidentally” detected by producing false positive results in non-treponema tests (RPR, VDRL) for syphilis
