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Serology > AutoImmune Disease II > Flashcards

AutoImmune Disease II Flashcards

1
Q

Anti-native DNA (nDNA) test:

- describe the test antigen

A

Crithidia luciliae, a flagellated parasite with kinetoplast that is rich in nDNA

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2
Q

Anti-native DNA (nDNA) test:

- Indicate which antibodies are detected

A

detects antibodies against double stranded DNA

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3
Q

Anti-native DNA (nDNA) test:

- appearance of a positive

A

Fluorescence of the kintoplast is a positive result (dot farthest away from flagella)

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4
Q

Anti-native DNA (nDNA) test:

- appearance of a negative

A

No fluorescence of kinetoplast (negative for anti nDNA)

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5
Q

Anti-native DNA (nDNA) test:

- Explain how test is used diagnostically

A

Positive result does not confirm SLE but nDNA antibodies are often found in SLE patients

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6
Q

Anti-native DNA (nDNA) test:

- What staining pattern should Anti nDNA antibodies produce

A

Either homogeneous or peripheral staining

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7
Q

Antibodies Against Extractable Nuclear Antigens (ENA)

- List antibodies that are detected

A

Smith, RNP, SS-A, SS-B, Jo-, Scl-70, and others (centromere B, chromatin, ribosomal P)

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8
Q

Antibodies Against Extractable Nuclear Antigens (ENA)

- antigen used in detecting antibodies

A

Same antigen as nDNA test: Crithidia luciliae ???

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9
Q

Antibodies Against Extractable Nuclear Antigens (ENA)

- Expected pattern in the ANA and the disease association for Smith

A

Speckled

SLE

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10
Q

Antibodies Against Extractable Nuclear Antigens (ENA)

- Expected pattern in the ANA and the disease association for RNP

A

Specked

SLE

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11
Q

Antibodies Against Extractable Nuclear Antigens (ENA)

- Expected pattern in the ANA and the disease association for SS-A

A

Speckled

Sjogrens

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12
Q

Antibodies Against Extractable Nuclear Antigens (ENA)

- Expected pattern in the ANA and the disease association for SS-B

A

Specked

Sjogrens

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13
Q

Antibodies Against Extractable Nuclear Antigens (ENA)

- Expected pattern in the ANA and the disease association for Scl-70

A

Homogeneous or Nucleolar

Scleroderma (PSS)

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14
Q

Antibodies Against Extractable Nuclear Antigens (ENA)

- Expected pattern in the ANA and the disease association for Jo-1

A

Cytoplasmic

Polymyositis

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15
Q

At IUHPL Serology Lab ENA Antibodies are detected by what? Principle?

A

Bioplex Multiplex Bead System

  • patients antibodies bind to corresponding beads
  • Labeled anti-IgG binds to antibody-coated beads
  • analyzed by laser interrogation by the instrument
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16
Q

Advantages and Disadvantages of using only an outdated system for ANA testing

A

Advantage: efficient, little expertise
Disadvantage: only Ab’s against Ag’s used in test system will be detected. Others will be missed

17
Q

How does the staining patterns of AMA and ASMA differ from those produced by by ANA’s in the ANA test

A

These produce cytoplasmic fluorescence in the ANA test

18
Q

What autoimmune diseases are characterized by presence of AMA and ASMA antibodies?

A

Liver Problems: primary biliary cirrhosis, chronic active hepatitis and others

19
Q

How are assays for AMA performed?

A

Indirect immunofluorescnce where rabbit kidney cells are the antigen

20
Q

How are assays for ASMA performed?

A

Indirect immunofluorescnce where tissue sections of rat stomach are the antigen

21
Q

Anti-cardiolipin antibodies

- what conditions are these antibodies found

A
  • autoimmune disorders
  • infections
  • neoplastic conditions
  • drug-induced lupus syndromes
  • often associated with recurrent episodes of arterial or venous thrombosis and recurrent miscarriages
22
Q

Anti-cardiolipin antibodies

- define antiphospholipid antibodies

A

A heterogenous group detected in a variety of patients with a variety of disorders

23
Q

Anti-cardiolipin antibodies

- How are they indicated in the lab

A

Individual tests are done and clotting test for “lupus anticoagulant” are done

24
Q

Anti-cardiolipin antibodies

- what is the effect of these on the non-treponemal tests for syphilis

A

They are “accidentally” detected by producing false positive results in non-treponema tests (RPR, VDRL) for syphilis

Serology (17 decks)

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