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IGCSE Biology > ATP > Flashcards

ATP Flashcards

(food tests)

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1
Q

What are the 4 food tests?

A

Iodine for starch
Benedict for reducing sugars
Biuret for Protein
Ethanol for Lipids

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2
Q

What is the negative color for Iodine test for starch

A

Yellow/Brown

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3
Q

What is the positive color for Iodine test for starch

A

Black

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4
Q

What is the negative color for Benedict test for sugar/glucose

A

light blue

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5
Q

What is the positive color for Benedict test for sugar/glucose

A

Range of colors
green- trace
yellow- low
orange- moderate
brick red- high

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6
Q

What is the negative color for Ethanol test for Lipids

A

colorless

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7
Q

What is the positive color for Ethanol test for Lipids

A

cloudy emulsion

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8
Q

What is the negative color for Biuret test for Protein

A

blue

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9
Q

What is the positive color for Biuret test for Protein

A

Purple

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10
Q

What does Iodine test for?

A

Starch

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11
Q

What does Biuret test for?

A

Protein

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12
Q

What does Emulsion (ethanol) test for?

A

Lipids (fats)

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13
Q

What does Benedict test for?

A

Reducing Sugar/ Glucose (concentration)

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14
Q

Method for Iodine test for starch

A
  1. Place food samples on spotting tile
  2. Number each sample
  3. Add two drops of iodine solution to each sample
  4. Record color of solution
    -safety: safety goggle + lab coat
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15
Q

Method for Biuret test for Protein

A
  1. Place sample food in test tubes and number them
  2. Add 2 cm^3 (20 drops) of biuret reagent to samples
    (if food is dry add 5 drops of distilled water
  3. Record color of reagent
    -safety: safety goggles + lab coat
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16
Q

Method for Emulsion (ethanol) test for Lipids (fats)

A
  1. Place food samples in a test tube and number them
  2. Add 2 cm^3 (20 drops) of ethanol to the food samples
  3. Shake the tube for 10 seconds
  4. Pour sample into tube of distilled water
  5. Record the color of reagent
    safety: safety goggles + lab coat
17
Q

Method for Benedict’s test for reducing sugars

A
  1. Place food samples in a boiling tube and number them
  2. Add 2 cm^3 (20 drops) of Benedict reagent to food samples
  3. Place the tube into a boiling water bath (carefully)
  4. Leave for 3-4 minutes
  5. Remove carefully with a test tube holder
  6. Record color of the reagent
    -safety: safety goggles + lab coat + avoid contact with skin

IGCSE Biology (7 decks)

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