ATP Flashcards
(food tests)
What are the 4 food tests?
Iodine for starch
Benedict for reducing sugars
Biuret for Protein
Ethanol for Lipids
What is the negative color for Iodine test for starch
Yellow/Brown
What is the positive color for Iodine test for starch
Black
What is the negative color for Benedict test for sugar/glucose
light blue
What is the positive color for Benedict test for sugar/glucose
Range of colors
green- trace
yellow- low
orange- moderate
brick red- high
What is the negative color for Ethanol test for Lipids
colorless
What is the positive color for Ethanol test for Lipids
cloudy emulsion
What is the negative color for Biuret test for Protein
blue
What is the positive color for Biuret test for Protein
Purple
What does Iodine test for?
Starch
What does Biuret test for?
Protein
What does Emulsion (ethanol) test for?
Lipids (fats)
What does Benedict test for?
Reducing Sugar/ Glucose (concentration)
Method for Iodine test for starch
- Place food samples on spotting tile
- Number each sample
- Add two drops of iodine solution to each sample
- Record color of solution
-safety: safety goggle + lab coat
Method for Biuret test for Protein
- Place sample food in test tubes and number them
- Add 2 cm^3 (20 drops) of biuret reagent to samples
(if food is dry add 5 drops of distilled water - Record color of reagent
-safety: safety goggles + lab coat
Method for Emulsion (ethanol) test for Lipids (fats)
- Place food samples in a test tube and number them
- Add 2 cm^3 (20 drops) of ethanol to the food samples
- Shake the tube for 10 seconds
- Pour sample into tube of distilled water
- Record the color of reagent
safety: safety goggles + lab coat
Method for Benedict’s test for reducing sugars
- Place food samples in a boiling tube and number them
- Add 2 cm^3 (20 drops) of Benedict reagent to food samples
- Place the tube into a boiling water bath (carefully)
- Leave for 3-4 minutes
- Remove carefully with a test tube holder
- Record color of the reagent
-safety: safety goggles + lab coat + avoid contact with skin