Assesment

Question 1

Innate immune system defects
Cellular→————-
humoral→……………….

Answer 1

􏱎Phagocytic cell defects

􏱎Complement defects

Question 2

(Quantitative)
Number of monocytes can be assayed by………………… using m.a.bs against specific cell markers present on monocytes ………..

Answer 2

flowcytometry/ CD14

Question 3

Fluorescence-activated cell sorting analysis (FACS) instrument used to…………….. and/or………….. live cells that express an antigen using fluorescent antibody specific it.

Answer 3

enumerate/separate

Question 4

Qualitative assay “Testing cell functions”

Tests for movement ………………………,………………………..

Tests for Phagocytosis………………..,…………………

NBT dye reduction test used to ……………………… & stimulated with…………………..

Normal cells produce superoxide that reduces NBT to……….

Dichlorofluorescein- Diacetate (DCF- DA) test: Cells that oxidize the DCF-DA􏱍………… fluorescent

Answer 4

Test for random motility
Test for Chemotaxis→ ability of cells to be attracted to a variety of chemotactic substances.

Phagocytosis assay / Generation of reactive Oxygen
intermediates (ROIs)

identify impaired respiratory burst activity

opsonized zymozan

blue

highly

Question 5

Complement defects

Quantitative assay»…………..or…………………………

Qualitative assay»……………………………

Answer 5

single radial immunodiffusion or ELISA.

Complement hemolytic activity (CH50)

Question 6

Adaptive immune system Humoral Immune system B cell defects

Quantitative assay» Number of mature B cells by ?

Answer 6

Flowcytometry (anti-cd 19 / anti-cd 20)

Question 7

Adaptive immune system >Humoral Immune system >B cell defects

Qualitative assay (B cell Function)
1- In vivo assay>……………………….
2- In vitro assay>………………………

Answer 7

Total immunoglobulins

B cell activation by mitogen(LPS)

Question 8

T-cell defects
􏱎Quantitative assay >…………………marker…….. & subset markers………….&……….
􏱎Qualitative (functional) assay>
In vivo by……….
In vitro by ……………………………,…………………….

Answer 8

Flowcytometry / anti -cd3 / anti-CD4 and anti-CD8

ELISA

Lymphocyte Culture Technique>
T cell activation by mitogen
Mixed lymphocyte culture (MLC)

Question 9

Cytotoxic T-lymphocyte Assay

• CD8+ T function can be assessed by their ability to…………………… target cells bearing foreign MHC class I mols.

Answer 9

kill radio labeled