Assays Flashcards
1
Q
What are microarrays used for?
A
gene interaction
2
Q
What is the ELISA used for?
A
- to detect serum antibody
- non-specific screening
- secondary test should be used such as RT PCR
3
Q
What is FACS used for?
A
Cell surface markings
- T cell subsets and determine clonality
- requireds intact cells
*what is this cell im interested in
4
Q
What is FACS used for?
A
Cell surface markings
- T cell subsets and determine clonality
- requires intact cells
*what is this cell im interested in
5
Q
What does RT PCR look for?
A
Highly specific microbial antigen or marker
6
Q
What is serum protein electrophoresis used for?
A
detect presence and clonality of antibodies
-spike is one expanded clone of plasma-cells making one type of antibody
7
Q
What is serum protein electrophoresis used for?
A
detect presence and clonality of antibodies
-spike is one expanded clone of plasma-cells making one type of antibody-malignancy of plasma cell-multiple myeloma
