Arboviruses 3 (Reoviridae) + Rhabdo/Filo

Question 1

Reoviridae genus

Answer 1

Coltivirus: Colorado Tick Fever Virus

Question 2

Coltivirus structure

Answer 2

• 12 Double stranded RNA segments
• NON enevloped
• icosahedral

Question 3

Coltivirus replication cycle (5 steps)

Answer 3

1. endocytosed, enters lysosome
2. core particle primary transcript
3. Translated into +/- strands in cytoplasm
4. replicase creates + strand from the - one
5. capsid assembly and release

Question 4

Colorado tick fever is a ____, _____ illness

Answer 4

nonspecific, febrile

Question 5

Coltivirus clinical findings (4)

Answer 5

1. Tick bite
2. Saddleback Fever
3. Flu-like symptoms
4. Transient Rash

(Complication of Encephalitis or pericarditis)

Question 6

Coltivirus infects _______ cells.

What effect does this have?

Answer 6

Bone Marrow cells

this halts development of blood cell types (PMN, eos, baso’s)

Question 7

When do Coltivirus symptoms occur

Answer 7

4-5 days after tick bite

Question 8

Significance of the first two weeks of Coltivirus infection

Answer 8

free virus can be found in the blood

after which it circulates within erythroblasts

Question 9

When does Coltivirus antibody appear?

Answer 9

two weeks after symptom onset

Question 10

Coltivirus lifespan in blood

Answer 10

can be isolated for 6 weeks

BUT can live in RBC for life of the cell

Question 11

Coltivirus resevoir and transmitter

Answer 11

Squirrel/rodent resevoir

Wood Tick is transmitter

Question 12

What tick spreads Coltivirus?

Answer 12

Rocky Mountain wood tick

(Dermacentor andersoni)

Question 13

90% of Coltivirus cases happen during…

Answer 13

april-july

Question 14

Coltivirus control

Answer 14

stay away from ticks

check yo pets

get out of your skin if embedded

Question 15

Filoviridae diseases

Answer 15

Ebola

Marburg

Question 16

Characteristics that filoviridae share

Answer 16

Genome structure

Zoonotic

Damage the microvasculature = hemorrhage

Question 17

Filovirus organism structure

Answer 17

ss (-) RNA

Encodes 7 proteins

Enveloped filaments

Helical

Question 18

Filovirus nucleocapsid enclosed in an envelope that contains…

Answer 18

one glycoprotein

Question 19

Filovirus replicates in the

Answer 19

cytoplasm

Question 20

Filovirus (ebola) causes ______ infection

Answer 20

hemorrhagic

Question 21

Ebola rash appearance and time

Answer 21

macropapular, appears at 5 days

Question 22

Signs of recovery occur at day ____ and are concurrent with…

Answer 22

Day 7

With antibody response

Question 23

Ebola transmission and reservoir

Answer 23

Unknown

possibly bats

Question 24

Major characteristic of Ebola (filo) infection is…

Answer 24

destruction of the immune system

especially the breakdown of endothelial cells

Question 25

Ebola cell damage due to ____

Answer 25

Cytokine release from Monocytes

Question 26

Ebola cytokine dysregulation promotes ______ and ______

Answer 26

hemorrhage + vasomotor collapse

Question 27

Ebola vector

Answer 27

Not identified

Question 28

Rotavirus serogroups

Answer 28

A-E

Question 29

Rotavirus genome and structure

Answer 29

nonenveloped

dsRNA

3 capsid layers + inner core

(Outer capsid VP4)

Question 30

Rotavirus VP4 protein is located on _____ and functions to aid _______

Answer 30

Outer capsid

host attachment

Question 31

Is rotavirus segmented or not?

Answer 31

Yes

11 segments of dsRNA

Question 32

Rotavirus transcription and translation occur in _______

Answer 32

Inner core = transcription

Cytoplasm = translation

Question 33

What is different about the replication of Rotavirus?

Answer 33

replicates in progeny core

uses positive RNA template

Question 34

Rotavirus release from cell, two steps…

Answer 34

buds into ER

release by cell lysis

Question 35

Rhabdovirus genus

Answer 35

Lyssavirus

Question 36

Rhabdo shape and structure

Answer 36

bullet shaped helical, enveloped

ss (-) RNA

Question 37

Rhabdo: associated proteins

Answer 37

NPMGL

(nucleo, phospho, matrix, glyco, polymerase)

Question 38

Rhabdo replication

Answer 38

1. Binds using G protein
2. Pinocytosis into cytoplasm
3. Endosome fusion and release RNP into cytoplasm
4. L gene (pol-ase) makes 5 mRNAs + 1 full (+)RNA
5. Translation of NPMGL (G = processed in ER and Golgi)
6. +RNA serves as templates for full length (-) RNA
7. Assemble NPL around RNP core, M forms capsule (matrix)
8. Bud from PM and release

Question 39

Rhabdo periods (and # of days for each)

Answer 39

Incubation (20-90)

Prodromal (2-10)

Neurological (2-7)

Question 40

Rhabdo travels via..

Answer 40

retrograde axonal transport

Question 41

Rhabdo pathogenesis duration depends on…

Answer 41

size & site of exposure

human/animal immunity

Question 42

Rhabdo replicates in _____

Answer 42

muscle at the site of innoculation

Question 43

Rhabdo concentrates in ______

Answer 43

salivary glands

Question 44

common reservoirs for rhabdo

Answer 44

bats, foxes, raccoons, skunks

Question 45

Rhabdo Dx

Answer 45

Histopathology

(also electron micro,

direct elisa/immunochemistry)

Question 46

signs of rhabdo in histopathology are… (5)

Answer 46

1. mononuclear infiltration
2. perivascular cuffing (lymph/PMN)
3. Lymphocytic foci
4. Babes nodules
5. NEGRI BODIES

Question 47

Rhabdo vaccine is unique in that it is adminitered

Answer 47

Post exposure

Question 48

Other measures taken after exposure to rhabdo (other than post exposure vax)

Answer 48

Clean site with soap and water

Question 49

Rhabdo vax administration schedule after exposure?

Answer 49

Days - 0, 3, 7, 14, 28

Question 50

Is there a pre exposure rhabdo vaccine?

Answer 50

Yes

3 doses

Question 51

What is the Wisconsin rabies treatment protocol?

Answer 51

Ketamine

Ribavirin

Amantadine