Apoptosis Flashcards
1
Q
What happens in apoptosis?
A
The DNA fragments, the membrane blebs and the cell produces apoptotic bodies that produce “eat me signals” and the parts of the cell are then digested by phagocytes
2
Q
How is apoptosis involved in vertebrate limb development?
A
Massive apoptosis occurs in the mesenchyme of the interdigital spaces
3
Q
How can apoptosis be detected?
A
By TUNEL staining as chromatin become fragmented -the broken bits of DNA can be stained
4
Q
How is TGF-B involved in apoptosis?
A
PRomotes apoptosis
5
Q
Mutations in TGF-B often cause cancer; why is this?
A
They cant undergo apoptosis and also they dont recognise the inhibition that TGFB tries to do
6
Q
What are the steps involved in producing a mouse knock out gene?
A
- Mouse totipotent stem cells
- Cloned copy of target gene
- Engineer knockout construct
- Transform ES cells with construct
- ES cells into ouse blastocyst
- Blastocyst into uterus
- Grow chimera mouse
- Breed knockout form of chimera
