Apoptosis Flashcards
1
Q
2 key features of apoptosis
A
cell breakdown but
1) preserve membrane integrity
2) NO inflammatory response triggered
2
Q
Key intrinsic pathway mechanisms
A
- Antagonize Bcl2 (anti apoptotic)
- Activate Bax/Bak channels
- Leak cytochrome C
- Activate caspases
3
Q
Key extrinsic pathway mechanisms
A
- Fas/FasL; binding
2. Polymerize Caspase 8
4
Q
Differences between apoptosis and Necrosis in smear patterns on a gel
A
- Necrosis - smear - each bp = Dnase and endonuclease activity
- Apoptosis - nucleosomal breaks - regulated fragmentation - only endonuclease activity
