Apheresis - Tech Flashcards
What are the specific gravities of plasma, platelets, monocytes, grnaulocytes, and RBCs?
Plasma: 1.025-1.030 Platelets: 1.040 Monocytes: 1.070 Granulocytes: 1.090 RBCs: ~1.095
What filter is required to filter out platelets, RBCs, lymphs, and granulocytes?
Platelets: 3um
RBCs: 7um
Lymphs: 10um
Granulocytes: 13um
What is the main application of FILTRATION apheresis?
Plasma collection mostly, some platelet collection.
What apheresis instruments are approved for MNC collection?
Fenway AMICUS, COBE Spectra, Spectra Optia, Fresenius AS104 and COM.TEC
What apheresis instruments are approved for TPE?
COBE Spectra, Spectra Optia, Fresenius AS104/COM.TEC, Haemonetics MCS
What apheresis instruments are approved for RBC depletion?
COBE Spectra, Spectra Optia, Fresenius AS104/COM.TEC
What apheresis instruments are approved for leukapheresis/plateletpheresis?
COBE Spectra, Spectra Optia, Fresenius AS104/COM.TEC
What instruments are used for LDL pheresis?
Kaneka MA-03, B.Braun HELP system
What instruments are used for photopheresis?
THERAOKS UVAR and CELLEX systems
How does LDL pheresis work?
Kaneka system: Adsorption of positive LDL/VLDL/LpA onto dextran sulfate columns
HELP system: Dilute plasma with acidic heparin (sodium acetate) to precipitate positively charged proteins
What are the problems posed by rotary seals?
Contact activation of platelets, cleaning
What was the first device used for white cell removal? What for?
NCI-IBM blood cell separator, used to harvest granulocytes from CML patients for therapeutic use.
How can sealless continuous centrifugation be accomplished?
Counter-rotate the tubing in the opposite direction.
What does it mean for an instrument to be “one-stage” or “two-stage”?
Refers to the different radii of chambers that the blood products separate through. May be different chambers or a split-radius chamber.
What is the sieving coefficient?
In membrane filtration, refers to the degree of separation of plasma proteins.
