Antinuclear Antibodies (ANA Testing) Flashcards
What ANA pattern is associated with Anti-Histone antibody?
Homogenous (diffuse)
What ANA pattern is associated with Anti-ds DNA (double stranded DNA)?
Peripheral (Rim)
What ANA pattern is associated with Anti-RNA and Anti-ENA (extractable nuclear antigens)?
Speckled
What ANA pattern is associated with Anti-nucleolar RNA?
Nucleolar
What ANA pattern is associated with SLE?
Homogenous (main),
Peripheral/rim
Speckled
What ANA pattern is associated with Scleroderma (PSS)?
Speckled & Nucleolar
What ANA pattern is associated with rheumatoid arthritis (RA)?
Speckled
What ANA pattern is associated with MCTD?
Speckled
What ANA pattern is associated with Scleroderma?
Nucleolar and Speckled
What ANA pattern is associated with Sjogren’s?
Nucleolar
What antibody/antibodies is/are associated with SLE?
Anti-histone
Anti-ds DNA
Anti-RNA and Anti-ENA
What antibody/antibodies are associated with Scleroderma (PSS - Progressive Systemic Sclerosis)?
Anti-RNA and Anti-ENA
Nucleolar
What antibody/antibodies are associated with RA?
Anti-RNA and Anti-ENA
What antibody/antibodies are associated with MCTD?
Anti-RNA and Anti-ENA
What antibody/antibodies are associated with Sjogren’s syndrome?
Nucleolar
What disease(s) is associated with a homogenous/diffuse ANA pattern?
usually SLE
What disease(s) is associated with a peripheral/rim ANA pattern?
SLE
What disease(s) is associated with a speckled ANA pattern?
SLE, scleroderma (PSS), RA and MCTD
What disease(s) is associated with a nucleolar ANA pattern?
Scleroderma
Sjogren’s syndrome
How does the speckled ANA pattern differ from the centromere ANA pattern?
Speckled ANA pattern –> granular/speckled staining in the nucleus of the interphase cells
Absence of staining in the chromosomal area of the metaphase mitotic cells.
Centromere ANA pattern –> granular/speckled staining in the nucleus of the interphase cells AND metaphase mitotic cells.
What extractable nuclear antibody is highly specific for SLE but occurs in only 29-30% of cases?
Smith (Sm) antibody
What % of patients with SLE will test positive for ANAs?
95%
What type of antibodies are found in primary biliary cirrhosis?
Anti-mitochondrial antibodies
What ANA pattern occurs to any extractable nuclear antigen devoid of histones or DNA?
Speckled
Which method remains the “gold standard” for ANA detection?
Slide-based immunofluorescence assay (IF)
How would you determine if a homogenous or peripheral ANA pattern was due to Anti-ds DNA or Anti-DNP (Le Factor?)
Request Crithidia
Crithidia + = Anti dsDNA –> SLE
Crithidia - = Anti-DNP (Le Factor)
A Negative ANA pattern would rule out what?
SLE
What antibody is associated with Centromere (kinetechore) ANA pattern?
Anti-centromere (CREST)
