Antigen Recognition in the Adaptive Immune System

Question 1

receptors of Ag recognition

Answer 1

TCR

B cell receptors (soluble and membrane bound)

Question 2

forms of Ag recognized Bcell receptors(L) vs TCR(R)

linear or conformational forms?

Answer 2

s

Question 3

diversity of BCR(L) vs TCR(R)

Answer 3

Question 4

BCR (L) and TCR (R) Ag recognition mediated by?

Answer 4

Question 5

BCR (L) and TCR (R) signaling functions mediated by?

Answer 5

Question 6

BCR and TCR effector functions mediated by?

Answer 6

Question 7

Bcell receptors for antigens structure

Answer 7

surface bound Ig with light and havey chains as well as constant and variable regions

Question 8

variable regions of the Ig

Answer 8

sites to recognize the Ag, possess CDR and are towards the n terminus

Question 9

classes of Ig, based on?

Answer 9

based on heavy chains present

IgA/D/G/E/M

Question 10

Ig subtypes heavy chains, major secreted form and functions

Answer 10

Question 11

stimulated b cells express which Ig, what regions are altered/ stay the same?

Answer 11

switiches from IgM or D to IgG, A and E, constant regions are altered but variable regions remain the same to remain bound to Ag

Question 12

binding of Ig’s

Answer 12

non covalent, will recognize an eptiope
occurs at variable region
variable affintiys

Question 13

T cell receptors for antigens structure

Answer 13

b and a chains with variable (n term) and constant regions (c term)
non-soluble, only found in the membrane
variable regions contain 3 CDRs each (6)

Question 14

MHC presentation to TCR, which chains play a role

Answer 14

a chains 1 and 2 of MHC participate in Ag recognize and recognize TCR

Question 15

Ig vs TCR 
Ag binding regions
changes to constant region
affinity of Ag binding 
on rate and off rate

Answer 15

Question 16

Steps in maturation of lymphocytes

Answer 16

Question 17

Germline organization of antigen receptor gene loci

Answer 17

multiple chains loci with various coding regions

Question 18

Recombination and expression of immunoglobulin (Ig) genes

Answer 18

Question 19

variation in Ag recognition receptors

limited by? how can it be enhanced?

Answer 19

limited with gene number but enhanced with certain processes: recombination, splicing and repair

Question 20

Enzyme mediating recombination

Answer 20

use of enzymes to produce recombination and DNA breaks that can be fixed thru filling or removal of overhang resulting in new sequences
can also lead to frame shift mutations that can result in a non-functional receptor

Question 21

Steps in the maturation and selection of B lymphocytes
Ig DNA/RNA
Ig expression

Answer 21

Question 22

Steps in the maturation and selection of MHC-restricted T lymphocytes

Answer 22