ANTICOAGULANTS

Question 1

Most commonly used blood collection tube in the Hematology section

Answer 1

EDTA

Question 2

CBC is also called

Answer 2

Hemogram

Question 3

Hemogram or CBC includes

Answer 3

RBC parameters, WBC parameters, Platelet parameters

Question 4

Optimal anticoagulant concentration in EDTA

Answer 4

1.5 mg per mL of blood

Question 5

Action of EDTA

Answer 5

Chelation of calcium

Question 6

Blood specimens must be analyzed within how many hours of collection

Answer 6

6 (RT); 24 (if stored at 4 C)

Question 7

Blood smears should be made within

Answer 7

3 hours of collection

Question 8

EDTA causes swelling of platelets (causes approx. ____ increase in MPV during the first hour)

Answer 8

20%

Question 9

Tubes used for flow cytometry, plasma chem determination, OFT and BGA

Answer 9

Heparin

Question 10

Optimal anticoagulan concentration in Heparin tubes

Answer 10

15 to 20 units per mL of blood

Question 11

Action of heparin

Answer 11

Binds antithrombin (inhibits thrombin and FXa)

Question 12

Three heparin formulations:

Answer 12

Ammonium heparin
Sodium heparin
Lithium heparin

Question 13

Causes the least interference in chemistry testing

Answer 13

Lithium heparin

Question 14

Most widely used anticoagulant for plasma and whole blood chemistry tests

Answer 14

Lithium heparin

Question 15

Heparin causes cellular clumping which leads to____________________ and ___________________

Answer 15

Pseudoleukocytosis; pseudothrombocytopenia

Question 16

Used for coagulation tests

Answer 16

3.2% sodium citrate

Question 17

Critical ratio between anticoagulant and blood

Answer 17

1:9

Question 18

Action of 3.2% sodium citrate

Answer 18

Chelation of calcium

Question 19

Number of inversions for: blood culture tubes

Answer 19

8

Question 20

Number of inversions for: Citrated tubes

Answer 20

3 to 4

Question 21

Number of inversions for: Serum tubes

Answer 21

0 (glass)
5 (plastic)

Question 22

Number of inversions for: Heparinized tubes

Answer 22

8

Question 23

Number of inversions for: EDTA tubes

Answer 23

8

Question 24

Number of inversions for: Sodium fluoride tubes

Answer 24

8

Question 25

Yellow top

Answer 25

Blood culture

Question 26

Light blue top

Answer 26

Citrate

Question 27

Red top

Answer 27

Serum tubes

Question 28

Green top

Answer 28

Heparinized

Question 29

Lavender or pink

Answer 29

EDTA

Question 30

Gray top

Answer 30

Sodium fluoride

Question 31

SPS actions:

Answer 31

Prevents phagocytosis
Prevents complement activation
Neutralizes some antibiotics
Anticoagulant

Question 32

Tan top used for

Answer 32

Lead determinations

Question 33

Royal blue used for

Answer 33

Toxicology, nutritional chemical determination, trace element determination

Question 34

White top used for

Answer 34

Molecular diagnostic tests

Question 35

Black top used for

Answer 35

Westergren ESR

Question 36

Pink top used for

Answer 36

Blood bank tests, whole blood determination

Question 37

CTAD tubes used for

Answer 37

Coag tests, platelet function assays