ANTICOAGULANTS Flashcards
Most commonly used blood collection tube in the Hematology section
EDTA
CBC is also called
Hemogram
Hemogram or CBC includes
RBC parameters, WBC parameters, Platelet parameters
Optimal anticoagulant concentration in EDTA
1.5 mg per mL of blood
Action of EDTA
Chelation of calcium
Blood specimens must be analyzed within how many hours of collection
6 (RT); 24 (if stored at 4 C)
Blood smears should be made within
3 hours of collection
EDTA causes swelling of platelets (causes approx. ____ increase in MPV during the first hour)
20%
Tubes used for flow cytometry, plasma chem determination, OFT and BGA
Heparin
Optimal anticoagulan concentration in Heparin tubes
15 to 20 units per mL of blood
Action of heparin
Binds antithrombin (inhibits thrombin and FXa)
Three heparin formulations:
Ammonium heparin
Sodium heparin
Lithium heparin
Causes the least interference in chemistry testing
Lithium heparin
Most widely used anticoagulant for plasma and whole blood chemistry tests
Lithium heparin
Heparin causes cellular clumping which leads to____________________ and ___________________
Pseudoleukocytosis; pseudothrombocytopenia
Used for coagulation tests
3.2% sodium citrate
Critical ratio between anticoagulant and blood
1:9
Action of 3.2% sodium citrate
Chelation of calcium
Number of inversions for: blood culture tubes
8
Number of inversions for: Citrated tubes
3 to 4
Number of inversions for: Serum tubes
0 (glass)
5 (plastic)
Number of inversions for: Heparinized tubes
8
Number of inversions for: EDTA tubes
8
Number of inversions for: Sodium fluoride tubes
8
