Antibodies

Question 1

Antibodies

Answer 1

Proteins that are part of specific immune system
Made in response to something detected as foreign
Specific: each Ab recognizes a distinctive antigen
Antigen binding sites: hand-like parts of Ab that attach to foreign bodies
Fc region: area of Ab at bottom on which fluorescent tags can be applied

Question 2

Antigen

Answer 2

Target to which antibody binds

Triggers immune response

Question 3

Epitope

Answer 3

Part of antigen to which antibody binds

Question 4

Raising antibodies

Answer 4

Purify protein
Inject protein into host
Animal’s immune system will recognize protein as foreign
Animal produces antibodies
Blood drawn from animal will contain desired antibody

Question 5

Monoclonal antibodies

Answer 5

Collection of identical antibodies

Question 6

Polyclonal antibodies

Answer 6

Collection of monoclonal antibodies

Each group has different binding sites

Question 7

Hybridoma cell

Answer 7

B cell injected with antigen A makes anti-A antibody but can’t divide forever
Tumor cell from cell culture divides indefinitely but doesn’t make any antibodies
Fuse B cell with tumor cell: hybrid cell makes anti-A antibody indefinitely

Question 8

Uses of antibodies in cell studies

Answer 8

Determine how many of a certain cell type are in a population of cells
Look for structural changes in a protein
Measure amount of protein in a sample
Drug delivery
Locate protein
Purify protein