Amniotic Fluid

Question 1

Anti-hCG source

Answer 1

Rabbit

Question 2

hCG peaks during

Answer 2

1st Trimester of Pregnancy

Question 3

Priciple of Home-based HCG Pregnancy test Kit

Answer 3

Enzyme Immunoassay

Question 4

Cut-off point for pregnancy test kit

Answer 4

25 mIU/mL

Question 5

Ultimate source of amniotic fluid water and solutes, and is a product of fetal metabolism

Answer 5

Placenta

Question 6

primary function of Amniotic fluid

Answer 6

Cushion for the fetus
Stabilizes temperature
Allows fetal movement
Proper kung development

Question 7

Amniotic fluid volume is from

Answer 7

Fetal urine and lung fluid

Question 8

Normal Amniotic fluid volume in 3rd trimester

Answer 8

800 - 1200 mL

Question 9

During the 1st trimester 35mL of AF is derived from

Answer 9

Maternal circulation

Question 10

Fetal urine is the major contributor to the AF volume AFTER the

Answer 10

1st Trimester

Question 11

Placed on ICE DELIVERY, kept REFRIGERATED or FROZEN
filtration prevents loss of PHOSPHOLIPIDS

Answer 11

Test for FETAL LUNG MATURITY

Question 12

Kept at ROOM TEMPERATURE or at 37C

Answer 12

Test for CYTOGENETICS STUDIES

Question 13

Protected from Light

Answer 13

Test for HDN

Question 14

Detects premature rupture of membranes, also used to diagnosed EARLY PREGNANCY

Answer 14

Fern Test

Question 15

Colorless- Pale Yellow

Answer 15

Normal

Question 16

Traumatic tap, abd. Trauma, Intra-amniotic hemorrhage

Answer 16

Blood-Streaked

Question 17

HDN Bilirubin

Answer 17

Yellow

Question 18

Meconium 1st fetal bowel movement

Answer 18

Dark-Green

Question 19

Fetal Death

Answer 19

Dark Red - Brown

Question 20

Ratio of Mature Fetal Lungs

Answer 20

> = 2.0

Question 21

CANNOT be performed in the presence of Blood and Meconium

Answer 21

L/S Ratio

Question 22

Reference method for Alveolar Stability

Answer 22

Lecithin

Question 23

Serves as a control due to constant production

Answer 23

Sphingomyelin

Question 24

Amniostat FLM is an immunologic test for

Answer 24

Phosphatidylglycerol (PG)

Question 25

NOT AFFECTED by Blood or meconium; production of PG is delayed among diabetic Mothers

Answer 25

Amniostat-FLM

Question 26

Reagent Used in Foam stability/ Shake test

Answer 26

95% Ethanol

Question 27

Measures by Fluorescence polarization

Answer 27

Microviscosity

Question 28

Produced srufactants stored in te form of lamellar bodies

Answer 28

Type II pneumocytes

Question 29

Lamellar Body Count considered to have Adequate FLM

Answer 29

> 32,000 /uL

Question 30

LBC can be done using IMPEDANCE or

Answer 30

Optical scatter

Question 31

O.D. of _____ is equivalent to L:S ration of >=2.0 and the presence of PG

Answer 31

> =0.150

Question 32

test for fetal age prior to 36 weeks of gestation

Answer 32

1.5 to 2.0 mg/dL amniotic fluid creatinine

Question 33

A test for HDN which is the OLDEST ROUTINE lab test on amniotic fluid

Answer 33

O.D. 450

Question 34

Normal absorbance of Amniotic Fluid

Answer 34

^ at 365nm < at 550nm

Question 35

Non-affected or Mildly affected fetus

Answer 35

Zone I

Question 36

Moderately affected fetus, requires careful monitoring

Answer 36

Zone II

Question 37

Severly affected fetus, requires intervention: labor or EXCHANGE TRANSFUSION

Answer 37

Zone III

Question 38

What is used to extract or remove hemoglobin to remove interference

Answer 38

Chloroform

Question 39

it shows A450 values from 14-40 weeks of gestation

Answer 39

Queenan curve (Mod. Liley curve)

Question 40

Screening test for NTD

Answer 40

AFP

Question 41

Confirmatory test for NTD

Answer 41

Acetylcholinesterase

Question 42

It is the major protein produced by the FETAL LIVER during early gestation (prior to 18 weeks)

Answer 42

AFP