Ai Flashcards
1
Q
How can semen be stored for AI?
A
. fresh
. fresh-extended
. extended-chilled
. extended-frozen-thawed
2
Q
What features can you use to determine sperm quality?
A
.Gross abnormality? . How many? . Motility? . Morphology? . Can sperm fertilise?
3
Q
How will a dead sperm appear when stained with nigrosin:eosin stain?
A
Dead sperm with damaged membranes allow eosin to
penetrate so the sperm will appear pink.
Sperm with intact membranes exclude eosin so sperm will remain unstained (white)
4
Q
What would a small circle on the tail of spermatid be called?
A
A cytoplasmic droplet.
5
Q
How is sexing semen accomplished?
A
The sexing is based on a separation of sperm based on their DNA content
.X chromosomes have more DNA than Y
chromosomes (3-4% more DNA)
.Separation is obtained by labelling the sperm
with a DNA florescent dye and sorting by flow
cytometry.
