Additional Tests

Question 1

Bone Marrow Evaluation-Why?

Answer 1

persistent unexplained pancytopenia, neutropenia
thrombocytopenia and non regenerative anemia
immature cells/neoplastic/parasitic infection

Question 2

Collection Supplies: Bone Marrow

Answer 2

EDTA syringe
bone marrow needle
No 11 scalpel blade
12 slides
sutures

Question 3

VT role: Bone Marrow Collection

Answer 3

restraint
general anesthesia
sedation
local anesthesia

Question 4

Aspiration Biopsy

Answer 4

head of humerus or femur
rotate and apply pressure
attach syringe and aspirate

Question 5

Core Biopsy

Answer 5

better sample quality
similar to aspirate-rotate needle to cut bone from core
use diff site if also done aspiration

Question 6

Smear Prep

Answer 6

mix immediately with EDTA
aspirate and prep like blood smear
rapidly air dry
Romanowsky (Diff Quick) stain-longer b/c thicker

Question 7

Evaluating Blood Films: Normal Values

Answer 7

Juvenile: 75% nucleated cells, 25% fat
Adult: 50% nucleated cells, 50% fat
Geriatric: 25% nucleated cells, 75% fat

Question 8

Slide Exam: 10x

Answer 8

number and distribution of cells

(high, med, low cellularity)

Question 9

Slide Exam: 40x

Answer 9

general evaluation

Question 10

Slide Exam: 100x

Answer 10

cell differential

Question 11

myeloid/erythroid ratio

Answer 11

example:
M=550, E=450
ration=550:450 or 1.22:1

Question 12

normal myeloid/erythroid ratio

Answer 12

close 1:1

Question 13

abnormal myeloid/erythroid ratio

Answer 13

increase in WBC, decrease in RBC

or opposite

Question 14

abnormalities: classification

Answer 14

cell number

cell morphology

Question 15

abnormalities: charachterized

Answer 15

hypercellular
hypocellular
absent-aplastic

Question 16

Erythrocyte Sedimentation Rate

Answer 16

rate RBCs fall in their own plasma

altered some diseases

Question 17

Erythrocyte Sedimentation: manual method

Answer 17

calibrate tube with EDTA
place perpendicular in rack
sit at room temp 60 min (20 min in horses)
record level at top RBC column

Question 18

Erythrocyte Osmotic Fragility

Answer 18

test ability to withstand hemolysis
in varying concentrations in saline
referral or research centers