Acute Leukemias

Question 1

M0 (AML)

Answer 1

-Stem Cell Leukemia
-[CD34+/- , HLA-DR+, CD117+/-] CD13+
-MPO: =
-SBB: =

Question 2

M1 (AML w/o maturation)

Answer 2

-90% myeloblasts
-Auer rods +
[CD13+, CD33+], CD34+, CD117+
-MPO: +
-SBB: +

Question 3

M2 (AML w/ maturation)

Answer 3

• <20% monocytic lineage
  -Auer rods +
  -Dysplasia +
  CD13, CD33
  -AML with t(8;21)

Question 4

M3 (APL)

Answer 4

-Promyelocytes
-DIC
-t(15;17) , PML-RARA
-Hypergranular variant (Most common); extremely heavy granulation, >30% myeloblasts/abnormal pro’s, auer rods+, faggot cells.
-Microgranular promyelocytic leukemia: granules are so small

Question 5

M4 (AMML)

Answer 5

-t(16;16)
-↑ WBC, Serum lysozyme
-↓ PLT

-Monocytic: >20% monos, promonos
-Monoblasts: MPO, SBB, NASDA +
-CD4, CD14

-Myeloid: 20-80%
-Myeloblasts: MPO, SBB, NASDA +/-
-CD13, CD33

Question 6

M5 (AMoL)
a/b

Answer 6

-80% monocytic origin
-↑ WBC
-DIC
-NSE+ (80%)
-SBB +/-
-CD14, CD4

Question 7

M6 (erythroleukemia)

Answer 7

-Di Guglielmo syndrome
-↓ M:E, 1:2-1:4
-Affected chromosomes 5&7
-PAS+, NSE+, NASDA+/-

-↓WBC, PLT
-Ringed sideroblasts
-H-J present
-NRBC’s present

Question 8

M7 (AMKL)

Answer 8

-Megakaryoblasts/Megakaryocytes
-MPO, PAS, NASDA, NSE +
-t(1;22)
-↑ reticulum fibrosis
-Leukopenia