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Hematology > Activity 3: Specimen Collection and Preparation of Samples > Flashcards

Activity 3: Specimen Collection and Preparation of Samples Flashcards

1
Q

needle gauge: orange

A

25

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2
Q

needle gauge: purple

A

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3
Q

needle gauge: blue

A

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4
Q

needle gauge: black

A

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5
Q

needle gauge: green

A

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6
Q

needle gauge: yellow

A

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7
Q

needle gauge: cream

A

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8
Q

needle gauge: pink

A

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9
Q

needle gauge: red-purple

A

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10
Q

needle gauge: white

A

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11
Q

Order of Draw for syringe and ETS

A
  1. blood culture
  2. sodium citrate tubes
  3. serum tubes
  4. heparin tubes
  5. EDTA tubes
  6. Glycolytic inhibitors
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12
Q

order of draw for capillary tubes

A
  1. blood gas
  2. slides
  3. EDTA
  4. heparin
  5. glycolytic iinhibitor
  6. serum
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13
Q

additive and use: yellow top

A

sodium polyanethol sulfonate (SPS) for blood culture
acid citrate dextrose (ACD) for blood banking, HLA phenotyping, paternity tests

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14
Q

additive and use: light blue

A

3.2% sodium citrate or CTAD for coagulation determination

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15
Q

additive and use: black

A

3.8% sodium citrate for sedimentation rate

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16
Q

additive and use: gold

A

serum separator tube (SST) for routine chemistry and serology tests

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17
Q

additive and use: red glass

A

no additive for serology and chemistry

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18
Q

additive and use: red plastic

A

clot activator for routine serology and chemistry

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19
Q

additive and use: orange

A

thrombin-based clot activator for STAT serum chemistry determination

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20
Q

additive and use: light green

A

plasma separator tube with lithium heparin for plasma chemistry determinations

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21
Q

additive and use: green

A

sodium or lithium heparin for plasma chemistry

22
Q

additive and use: lavender glass

A

liquid K2EDTA for hematology

23
Q

additive and use: lavender plastic

A

spray K3EDTA for hematology

24
Q

additive and use: pink plastic

A

spray K3 EDTA for bloodbanking, donor screening and crossmatching

25
Q

additive and use: tan

A

K2 EDTA for lead determination

26
Q

additive and use: white

A

K2EDTA with gel for PCR and DNA amplification

27
Q

additive and use: gray

A

potassium ocalate and sodium fluoride for glucose determinations

28
Q

interferences in coagulation testing

A
  1. prolinged tourniquet
  2. contamination with tissue thromboplastin
  3. contact with inappropriate specimen container
  4. improper temperature
  5. hemolysis (like thromboplastin)
29
Q

mechanism of inhibition of sodium citrate

A

bind to ionized calcium

30
Q

concentration of 3.2% Sodium citrate

A

0.109 M

31
Q

concentration of 3.8% sodium citrate

A

0.129 M

32
Q

increased hematocrit values or incomplete filling of the tube causes

A

increased clotting time

33
Q

formula of volume to be added to 0.5 mL 0.109 M sodium citrate

A

60 / (100- hct) x 4.5

34
Q

preferred anticoagulant for platelet counts

A

EDTA

35
Q

EDTA mechanism of action

A

chelation or binding of calcium ions

36
Q

optimal EDTA concentration

A

1.5 mg/ mL

37
Q

can be used for platelet retention test

A

heparin

38
Q

blood contact with glass surfaces affect what contact factors

A

Factors XI and XII (shortern intrinsic and extrinsic pathway, there fore use plastic, polystyrene or silicon coated)

39
Q

factors that will deteriorate in room temperature

A

labile factors V and VIII

40
Q

factors that are activated prematurely by the cold

A

factors VII and XI

41
Q

preserves labile factors V and VIII and sensitive to heparin therapy monitoring

A

trisodium citrate

42
Q

for people with normal hematocrits, ratio of blood to citrate is

A

9:1

43
Q

EDTA cannot be used for coagulation studies due to 2 reasons

A
  1. inhibits fibrinogen-thrombin reaction
  2. unstable factor V
44
Q

mechanism of action of heparin

A

acts with anti-thrombin III and inhibits reaction at all stages of coagulation

45
Q

pH increase by loss of CO2 can cause

A

prolonged clotting times

46
Q

for most coagulation tests, this specimen is required

A

platelet free plasma

47
Q

plasma should be immediately tested or if not

A

stored at refrigerator temperature for not more than 2 hrs

48
Q

samples should not be _____ if testing can be done within ____

A

frozen, 2 hrs

49
Q

ice particles form with slow freezing which will

A

denature clotting proteins

50
Q

if freezing is necessary, it should be done ____

A

rapidly at -20° C

51
Q

stable at 4 hours and survives repeated freezing and thawing

A

fibrinogen

Hematology (8 decks)

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