Activity 1: Lab Equipment for Coagulation Testing and Hemostasis

Question 1

receptacle for blood and othher reagents during hematological testing

Answer 1

test tube

Question 2

hematology tests that use test tubes

Answer 2

Clotting Time
Activated partial prothrombin time
Prothrombin time

Question 3

sizes of test tubes

Answer 3

13 x 100
12 x 75
10 x 75

Question 4

for holding objects under the microscope and preparing blood smears

Answer 4

glass slides

Question 5

hemostasis tests done in glass slides

Answer 5

clotting time (micro/slide method)

Question 6

size of glass slides

Answer 6

75 x 26 mm
~ 26 mm

Question 7

long cylindricap shaped glassware with graduations used to transfer a volume of liquid

Answer 7

pipette

Question 8

a pipette can transfer liquid with ranges

Answer 8

0.1 mL to 25 mL

Question 9

conical glass cylinder that fits into the compartment of a cylinder

Answer 9

graduated centrifuge tube

Question 10

hemostasis test that uses a graduated centrifuge tube

Answer 10

clot retraction test

Question 11

long, glass capillary pipette used for diluting blood

Answer 11

thoma pipette

Question 12

True or False: the graduations of a thoma pipette are equivalent that of a specific volume

Answer 12

False

Question 13

what are the parts of a thoma pipette

Answer 13

short narrow stem
bulb
long narrow stem

Question 14

The graduations of an RBC thoma pipette reach up to

Answer 14

101

Question 15

The graduations of an WBC thoma pipette reach up to

Answer 15

11

Question 16

a thick glass slide with H- shaped moat and 2 counting areas

Answer 16

hemocytometer

Question 17

hemocytometer with a silver mirror that increases contrast

Answer 17

Brightline

Question 18

sterile stainless steel pointed device for making small incisions for blood sampling

Answer 18

lancet

Question 19

impede flow of blood to easily palpate vein

Answer 19

tourniquet

Question 20

parts of a hypodermic needle syringe

Answer 20

needle
needle hub
barrel
plunger
leading and trailing ring

Question 21

parts of an evacuated tube system

Answer 21

two way needle
threaded hub with rubber sleeve
adapter

Question 22

ling rubber tubing that provides suction to a thoma pipette during dilution

Answer 22

sucking tube

Question 23

digital display for exact timing

Answer 23

stopwatch

Question 24

hemostasis tests that utilize a stop watch

Answer 24

clotting time
bleeding time
APTT
PT

Question 25

also used as a tourniquet

Answer 25

sphygmomanometer

Question 26

hemostasis tests that use a BP cuff

Answer 26

bleeding time capillary fragility test

Question 27

parts of a sphymomanometer

Answer 27

inflatable cuff manometer rubber bulb

Question 28

for magnifying appearance of specimen for counting and identification

Answer 28

compound light microscope

Question 29

metal chamber that providess constant temperature

Answer 29

water bath

Question 30

minimum and maximum settings of routine water bath

Answer 30

room temperature to 60° C

Question 31

temperature used for in vitro testing, replicating internal body conditions

Answer 31

37° C

Question 32

piston driven device used to transfer precise amounts of liquid

Answer 32

micropipette

Question 33

pipette ysed for manual cell counts

Answer 33

unopette system

Question 34

parts of an unopette system

Answer 34

shield pipette reservoir

Question 35

cleaning of test tube

Answer 35

boiled in water with 1-2% mild soap detergent for 30 mins and oven dried in < 140° C

Question 36

cleaning of glass slides

Answer 36

immersed in basin with warm water for a whole day, rubbed with lint free wipe or cotton swab and oven dried <80°C

Question 37

pipette cleaning

Answer 37

soaked in cleaning solution overnight and HcL if necessary, aspirate distilled water multipke times and chosen solution before using

Question 38

pipette maintenance

Answer 38

check for chips and calubrate regularly

Question 39

graduated centrifuge tubes cleaning

Answer 39

mild lab detergent solution and air dry

Question 40

thoma pipette cleaning

Answer 40

sodium hypochlorite or detergent overnight and dry through suction

Question 41

hemocytometer cleaning

Answer 41

10% bleach solution and water, strong acids or bases avoided. rinse with acetone and dried with cloth or soft wipe

Question 42

cleaning of sucking tube

Answer 42

wash with detergent and rinse with distilled water

Question 43

maintenance of sucking tube

Answer 43

avoid petroleum based products to prevent discoloration

Question 44

stopwatch cleaning

Answer 44

mild soap or lightly dampened cloth, avoid strong chemicals such as alcohol or acetone

Question 45

solution used to clean optical parts of the microscope

Answer 45

40% petroleum ether 40% ethanol 20% ether (96-97% ethanol dissolves the cement!)

Question 46

used to remove dust from microscope

Answer 46

came hair brush or blower

Question 47

used to remove pil residue from lenses

Answer 47

lens paper absorbent paper medical cotton wool

Question 48

protects microscope moving parts against corrosion

Answer 48

oil except vegetable oil (hardens)

Question 49

micropipette maintemamcd

Answer 49

calibrate every 3 months using dye solutions

Question 50

hemostasis tests that use water bath

Answer 50

PT APTT fibrinogen activity test