ABO Typing Flashcards
Who discovered the ABO?
Karl Landsteiner -1901
AB Ag is discovered by__________-
Adriano sturli and Von decastello
This is the most important blood group system
ABO Blood Group
TRUE OR FALSE:
Small amount of incompatible blood does not result to ITHR
FALSE
Even 0.1ml of ABO incompatible blood result to IHTR
The ABO gene is found in what chromosome?
Chromosome 9 (long arm) = 9q
What is the ISBT no. of ABO?
001
Who described the inheritance of ABO?
Bernstein
TRUE OR FALSE:
ABO gene is inherited from each parent
TRUE
In direct/foward grouping, what is the principle used?
Direct Agglutination/Hemeagglutination
What is being detected in Px’s sample in forward/direct typing?
Ag
What is the reagent used in forward/direct typing?
Known Anti-sera (Ab)
What is the specimen used in direct/forward typing?
Px’s RCS
What reagent is used to detect Weak A, B?
Anti-A,B
What is the technique used to produce monoclonal Abs?
Hybridoma Technology
What is the color of Anti-A?
Blue = asul
What is the color of Anti-B?
Yellow = banana
To produce larger agglutination, what monoclonal antibody is used?
IgM
TRUE OR FALSE:
Known antisera are commercially produced and can be produced in the lab
TRUE
In the direct/forward typing, if the Px has Anti-B, the Px’s blood type is ___________
A
In the direct/forward typing, if the Px has A Ag, the Px’s blood type will agglutinate in ___________
Anti-A and Anti-AB
In the direct/forward typing, if the Px has AB Ag, the Px’s blood type will agglutinate in ___________
Anti-A, Anti-B, Anti-AB
What is being detected in backward/indirect/reverse typing?
Antibodies
What is the reagent used in backward typing?
Known red cells
What is the specimen used in backward typing?
Serum
TRUE OR FALSE:
Plasma specimen can be used because it has fibrinogen
FALSE
It can cause formation of fibrin clots leading to false + results
Known O cells is used in backward typing because _____________
it is used to detect Bombay phenotype, and cold Ab/agglutinins
In backward/indirect typing, if the Px has Anti-B, it will agglutinate in __________________
Known B, and AB
In backward/indirect typing, if the Px has Anti-A, it will agglutinate in __________________
Known A, and known AB
In backward/indirect typing, if the Px has Anti A, Anti-B, Anti-AB it will agglutinate in __________________
Known A, B, and AB
In backward/indirect typing, if the Px has no Abs, it will agglutinate in __________________
Known O
Among asians, the most common blood type is _________
Type O
What is the universal donor for red cell?
Type O
What is the universal donor of plasma?
Type AB
What is the universal recipient of plasma?
Type O
What is the universal recipient of red cell?
Type AB
packed RBC is transfused if the Px ___________
has low Hgb or Hct
If Px has hypovolemia or DEC level of plasma, transfuse _____________
plasma
TRUE OR FALSE:
ABO Abs are detectable immediately at birth
FALSE - it is produced 37th day of life or 5-6weeks
TRUE OR FALSE:
ABO Abs are naturally occuring.It does not need piror exposure to be stimulated
TRUE
Which immunoglobulin can cross the placenta and could cause HDN?
IgG
What is the chemical reagents used for adsorption?
chloroquine diphosphate
What is the chemical reagents used for elution?
chloroquine diphosphate, digitonin acid, and heat
Define adsorption
It is the process of removing Abs in the Px’s serum using known red cell
Define elution
It is the process of dissociating the Ab in RBC surface
If a baby had HDN, the baby appears ___________
jaundice
What is the typing used for newborn and geriatrics?
Forward typing
What sample is used for the detection of ABO Ags in newborn?
cord blood through cordocentesis and percutaneous umbillical blood sampling
ABO Ags peaks at what age?
2-4yrs old
what substance is important for ABH Ag in red cell?
Type 2 precursor substance/ Paragloboside/ Oligosaccharide chain
