A&P 1 Unit 3 (Lab) [Intro to the Microscope]

Question 1

Ocular Lens

Answer 1

The lens through which you look to examine the slide.

Question 2

Objective Lens

Answer 2

Lenses with various powers of magnifications. 
Low: 4X
Medium: 10X
High: 40X
Oil Immersion 100X

Question 3

Stage

Answer 3

The surface on which the slide sits. Used to move the slide to the point of interest.

Question 4

Arm

Answer 4

Supports the body of the microscope and typically houses the adjustment knobs.

Question 5

Coarse Adjustment Knob

Answer 5

Large knob on the side of the arm and moves the stage up and down to change the distance of the stage from the objective lenses. Allows gross focusing of the image.

Question 6

Fine Adjustment Knob

Answer 6

Allows fine-tuning of the image’s focus.

Question 7

Lamp

Answer 7

Also called the illuminator, provides the light source. It rests on the base of the microscope.

Question 8

Iris Diaphragm

Answer 8

Adjustable wheel on the underside of the stage controls the amount of light allowed to pass through the slide.

Question 9

Body

Answer 9

Supports the eyepiece and the objective lenses.

Question 10

Condenser

Answer 10

A lens that serves to concentrate light from the illumination source that is in turn focused through the object and magnified by the objective lens.

Question 11

Iris Diaphragm Lever

Answer 11

Part of the condenser that slides back and forth to control the opening that allows light through.

Question 12

Pointer

Answer 12

Metal piece under the glass in the ocular lenses.

Question 13

Refraction

Answer 13

Bending of light

Question 14

Magnification

Answer 14

An apparent increase in the size of the object.

Question 15

Resolution

Answer 15

Minimum distance between 2 objects that the microscope can distinguish as separate objects. (Resolution of the light microscope is 0.2 µm)

Question 16

Focus

Answer 16

Ability to bring the image into clarity

Question 17

Depth of Focus

Answer 17

Thickness within the specimen that is in sharp focus.

Question 18

Porfocal

Answer 18

Quality of the microscope lenses that allows the focus to remain (about) the same when changing magnification.

Question 19

Magnification of Ocular Lens

Answer 19

10X on to the objective lenses. (Low: 4X=40X; Medium: 10X=100X; High: 40X=400X; Oil Immersion: 100X=1000X)

Question 20

Holding the Microscope

Answer 20

Use 2 hands to support the microscope when carrying it–one hand to hold the arm and the other hand to support the base.

Question 21

Lens Cleaning

Answer 21

Clean the lenses with lens paper only. Do not use paper towels or cloth to clean a lens, because this will scratch its surface.