A. 1) Transcription

Question 1

capping

Answer 1

RNA polymerase begins synthesizing an RNA transcript complementary to the template strand of the DNA. Synthesis is 5’—>3’. Other enzymes remove one (P) from 5’ end of RNA and add a GMP 5’—>5’

Question 2

where is the cap

Answer 2

at the 5’ end of the primary RNA transcript

Question 3

what is RNA splicing

Answer 3

eukaryotic genes are composed of expressed sequences (exons) and intervening sequences (introns). splicing removed the introns and join the exons

Question 4

what is alternate RNA splicing

Answer 4

primary mRNA can be spliced in diff ways to include diff exons and therefore encode diff proteins

Question 5

what is poly-adenylation

Answer 5

as the 3’ end of RNA is synthesized, a poly A polymerase uses ATP to add `200 AMP. No template is used. called poly A tail

Question 6

when is transcript called mRNA

Answer 6

once primary transcript is capped, spliced, and poly-adenylated. ready for export from nucleus