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Elsevier Hematology (RVSP) > 8. Cytochemical Stains > Flashcards

8. Cytochemical Stains Flashcards

1
Q

Stains lipids found in primary and secondary granules of granulocytic cells and in lysosomal granules of monocytes

A

Sudan black B (SBB)

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2
Q

Stains esterase enzymes found in neutrophils

A

Specific esterase (naphthol AS-D chloroacetate esterase)

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3
Q

Stains the esterase enzymes found in monocytes

A

Nonspecific esterase (α-naphthyl acetate/butyrate esterase)

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4
Q

Stains the isoenzymes of acid phosphatase

A

Tartrate-resistant acid phosphatase (TRAP)

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5
Q

Stains glycogen, mucoproteins, and high-molecular-weight carbohydrates

A

Periodic acid-Schiff (PAS)

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6
Q

Stains the enzyme in the secondary granules of mature neutrophils

A

Leukocyte alkaline phosphatase (LAP)

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7
Q

Stains DNA polymerase in cell nuclei

A

Terminal deoxynucleotidyl transferase (TdT)

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8
Q

Used to differentiate AML and ALL

A

MPO

SBB

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9
Q

Used to identify cells of myeloid in origin

A

Specific esterase

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10
Q

Used to diagnose AML of myeloid versus monocytic in origin

A

Nonspecific esterase

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11
Q

Positive diagnosis of hairy cell leukemia

A

TRAP

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12
Q

Used to diagnose AML of erythroid or megakaryoblastic in origin

A

PAS

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13
Q

Used for differentiation of CML and leukemoid reaction

A

LAP

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14
Q

LAP activity of CML

A

Low activity

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15
Q

LAP activity of leukemoid reaction

A

High activity

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16
Q

Used for identification of lymphoblasts in ALL

A

TdT

17
Q

Positive reaction in MPO

A

Myeloblasts

Promyelocytes

18
Q

Positive reaction in SBB

A

Myeloblasts

Promyelocytes

19
Q

Positive reaction in specific esterase

A

Myeloblasts

20
Q

Positive reaction in nonspecific esterase

A

Monoblasts

Monocytes

21
Q

Positive reaction in TRAP

A

Hairy cell lymphocytes

22
Q

Positive reaction in LAP

A

Neutrophils

23
Q

Positive reaction in TdT

A

Lymphoblasts

24
Q

Negative reaction in PAS

A

Normal erythroblasts

25
Q

Stains the enzyme found in primary granules of granulocytic cells

A

Myeloperoxidase (MPO)

Elsevier Hematology (RVSP) (12 decks)

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