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Bacteriology HO > 6/16 > Flashcards

6/16 Flashcards

1
Q

Taylorella

A

Reportable dz

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2
Q

Taylorella equigenitalis

A

gram neg

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3
Q

Taylorella equigenitalis species

A

horses
male shows no symptoms, no immunity
mare CEM immunity

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4
Q

CEM

A

contagious equine metritis - STD

only female has symptoms

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5
Q

Metritis

A

asymtomatic carriers highly contagious temporary infertility

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6
Q

poor environmental resistance

A

max 2 days in fridge- will die, care with sampling for dx

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7
Q

Taylorella

A

bacterial sampling of stallion

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8
Q

Taylorella equigenitalis sampling of stallion

A

prepuce
fossa glandis/ sinus urethralis
urethra
RUSH TO LAB- more than 2 days all the other bact will grow Taylorella will die

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9
Q

Taylorella equigenitalis sampling mare

A

sinussen

clitoris

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10
Q

Culture Taylorella equigenitalis

A

capnophilic long incubation

TAKE CARE OF YOUR SAMPLE

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11
Q

Dx Taylorella equigenitalis

A

PCR*

Biochemical testing

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12
Q

Prevention Taylorella equigenitalis

A

eliminate positive animals from breeding, carriers
hard to sanitize
vax poor

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13
Q

Taylorella equigenitalis uterine inf

A

remain carriers
antibiotics not very successful at clearing…will resolve on their own
self limiting

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14
Q

Brucella

A 
gram negative
small nonmotile
obligate symbiotic, resistant
obligate pathogen, facultative intracellular***
difficult to culture
REPORTABLE
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15
Q

B.melitensis

A

several animal species mainly goat and sheep

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16
Q

b. abortus

A

cattle

17
Q

b. suis

A

swine (as well as wildlife)

18
Q

Brucella

A

High genetic homology- creates problems with dx

19
Q

B.melitensis

A

sheep and goat

principle cause of human brucellosis

20
Q

B.abortus

A

many countries have eradicated,

very contagious

21
Q

Brucella serology testing

A

be aware of y.enterocolitica false positive

22
Q

spread of Brucella

A

abortion via female, highly contagious. Fetus and placenta contain huge amounts of bacteria

can spread through dogs and horses

23
Q

Brucella abortus

A

incidence in humans,
high prevalence in Mongolia
eradicated in the USA

24
Q

Brucella abortus pathogenesis

A

mucosa, oral, wounds
spread to regional lymph nodes then further spread to other LN (intracellular in macrophages for 1-6 wks) No symptoms no immune response, then to target organs (symptoms develop) repro organs, articulations

25
Q

Brucella abortus

A

calves that survive become carrier

dont drink raw milk in endemic areas

26
Q

Brucella abortus symptoms

A

intermittent fever 3-4 wks

27
Q

Brucella abortus virulence

A

cell wall w/ LPS
thicker peptidoglycan
erythritol- responsible for organ localization ( repro and mamm)

28
Q

smooth colonies

A

virulence increased
more pathogenic and zoonosis
not a stable phenotype
melitenis,

29
Q

immune rxn Brucella abortus

A

come late, cellular immunity 1st (abnormal)

when immunity goes down- pregnancy- bacteria start multiplication and release from cells, abortion or transfer to fetus

30
Q

vaccination Brucella abortus

A

need cellular immunity -

31
Q

dx Brucella abortus

A

slow agglutination
rapid agglutination - rose bengal
cft
ELISA
Skin test- swelling rxn because of cytokine production not recommended by OIE. EU regulation only to prove false positive rxns.
detect cellular immunity - IFN-gamma produced if cells have been primed ($$$)

Bacteriology

32
Q

culture Brucella abortus

A

grow on specific media
needs 10-20% co2
produce h2s
primary isolation 4-7d incubation

33
Q

false positives

A

b. suis
y. enterocolitica

Tests order

  1. saw
  2. elisa
  3. skin test
34
Q

Brucella suis

A

pigs

35
Q

Brucella suis

A

similar to abortus…. resistant in the environment
reportable
present in wildlife (boars) hunters often infected
eradicated in domesticated swine (us cananda eu)

36
Q

Biovars

A

5
difficult to discriminate
most pathogenic 1-3
different reservoirs, hare, reindeer, pigs
5 genetically similar to marine species
have been reported in cows, confused with abortus false pos.

37
Q

Brucella suis symptoms

A

reproduction system , orchitis, infertility, arthritis in PIGS
more pathogeneis for humans
all general symptoms long term illness
fever arthritis

38
Q

Brucella suis pathogenesis

A

skin oral nasal
localization in l. nodes in macrophages, antibodies produced later on
organ invation

39
Q

Brucella suis immunity

A

cellular humoral

carriers in macrophages

Bacteriology HO (6 decks)

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