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Bacteriology HO > 6/11 > Flashcards

6/11 Flashcards

1
Q

psuedemonas and mastitis

A

can cause mortality

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2
Q

pseudomonas aeruginosa in reptiles

A

necrotic stomatits. pneumonia, septicemia

secondary to poor housing

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3
Q

parrots and parrakeets pseudomonas aeruginosa

A

2ndary
eye
airway- pneumonia
origin drinking water (biofilm formation)

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4
Q

pseudomonas aeruginosa galliformes

A

water supply
localized in oviduct of turkeys if they dont die
contamination of eggs
pseudomonas enter eggs and make them explode via H2S gas production
neonatal mortality
complication of viral infection especially when the viral inf is treated with antibiotics

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5
Q

pseudomonas aeruginosa treatment

A

take away the cause
use antibiotics consider conditions and animal species.
consider culling (dairy cow)
careful with Blactams
use anti pseudomonas (cefoperazone)
most of the animals die (except with otitis externa)

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6
Q

Phage therapy

A

burn wound victims

local application strain specific

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7
Q

pseudomonas spp in cold blooded animals

A

fish: skin ulcer and septicemia

first thing: change water, clean environment, eliminate biofilm

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8
Q

Burkholderia

A 
gram -
aerobic
 rods 
catalase positive
previous had other names- taxonomical problems
environmental
lung inf
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9
Q

Burkholderia pathogenic species

A

b. mallei
b. pseudomallei
b. cepacia

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10
Q

Burkholderia mallei

A 
glanders
BSL3 agent
used in WWI- biological warfare
primarily EQUINE pathogen (also feline dog human) 
HAS TO BE DECLARED 
now mainly in asia and s. america
Horses travel!
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11
Q

Burkholderia mallei symtoms

A

nodules and ulcers , pyogranulomatous
orchitis
skin of resp tract
great variability in severity
ACUTE: fetal! fever nasal discharge, lyphadenitis
CHRONIC: you should not see- should be declared in chronic form. fever and respiratory skin abcesses
ZOONOTIC

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12
Q

Burkholderia mallei virulence

A

capsule - allows to survive in the environement

LPS

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13
Q

Burkholderia mallei transmission

A

ingestion inhalation wounds
many portals of entry
inhalation– high chance ofcontact

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14
Q

Burkholderia mallei pathogenesis

A

pharynx, wound

spread via lymphatic (nodules) or blood (lung liver spleen)

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15
Q

Burkholderia mallei pathology

A

nodular lesions composed of neutrophils fibrin and RBC
evolve when become more chronic
essential necrotic part
tx is difficult, AB do not penetrate the nodules easily surrounded by epithelial capsule

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16
Q

Burkholderia mallei typical symptom

A

NASAL DISCHARGE

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17
Q

Burkholderia mallei DX

A

isolation and ID
Serology- difficult
false positives (environmental, lots of epitopes)
complement fixation
intradermal test (mallein test) look for type 4 hypersensitivity (more sensitivity)

18
Q

Burkholderia mallei PX

A

NO VACCINES
Cull infected animals
avoid contact with endemic areas

19
Q

Burkholderia psuedomallei

A 
MELIODOSIS
re portable dz
saprophyte- in water rich environments
frequent inf after flooding swampy terrain
rice paddies
lives with environmental ameoba
wide host range
sheep goat rodent(reservoir)
tropical subtropical
20
Q

Burkholderia psuedomallei transmission

A

ingestion wound infection airbourne arthropod bite wound

21
Q

Burkholderia psuedomallei virulence

A

adhesins
capsule
T3SS and T4SS

22
Q

Burkholderia psuedomallei horses

A

mimics glanders: pseudoglanders

23
Q

Burkholderia psuedomallei in cattle

A

acute to chronic…. lungs joint uterus

24
Q

Burkholderia psuedomallei goats

A

more chronic dz

25
Q

Burkholderia psuedomallei swine

A

backyard swine, tropical

26
Q

Burkholderia psuedomallei DX

A 
environmental
 BSL3
purulent content swab
cultivation on blood mcconkey agar
CFT test
27
Q

Burkholderia psuedomallei prevention/ therapy

A

no vaccination
difficult to prevent water and soil contact
antimicrobials
most often end up culling

28
Q

Burkholderia mallei

A 
should not see (reportable)
be careful with traveling horses to endemic areas
false positives do exist 
confused with pseudomallei
difficult to contain
29
Q

Francisella

A

BSL3

30
Q

Francisella tularensis 3 subspecies

A

mediasiatica
holartica
tularensis

31
Q

Francisella tularensis

A 
gram negative
small
pleomorphic
nonmotile
contagious
obligate aerobic
facultative intracellular (hide in body, carriers) 
 very fastidious growth
32
Q

Francisella tularensis requires to culture?

A

SPECIFIC rich media chocolate agar thayer Martin agar

w/ supplement

33
Q

Francisella tularensis

A 
REPORTABLE
highly contagious 
prairie dogs from the US
wide rAange of animals
logomorphs- reservoir
34
Q

Francisella tularensis transmission

A

tick mosquitoes flies
contaminated waters
ingestion and infected prey
humans contact with wild life, inhalation (hunters)

35
Q

Francisella tularensis virulence

A

capsue
LPS
factors that promote intracellular survival in pagocytes, located on pathogenicity island

36
Q

Francisella tularensis spread

A

dz of Northern hemisphere
F. tularensis subs. tularensis- north america
holarctica- europe
mediasiatica- central asia

37
Q

Francisella tularensis lagomorphs

A

wild life dz

reservoirs

38
Q

Francisella tularensis pathogensis

A

always a systemic phase with multi organ failure

facultative intracellular- macrophages

39
Q

Francisella tularensis presentation

A

RARE
symptoms rarely seen- succumb fast
liver spleen lungs

40
Q

Francisella tularensis in humans

A

depends on mode of transmission and virulence of strain. American strain more virulent than european

41
Q

Francisella tularensis mode of transmission

A

ulceroglandular ( ulcerated cutaneous lesions with lyphadenopathy )
glandular (no skin lesions)
oculoglandular (conjunctivitis)
oropharyngeal( pharyngitis gastro enteritis)
inhalation
systemic

42
Q

TX And PX

A

antibiotics - not very resistant but need intracellular infusion (tetracyclines)
prevent ticks, eliminate contaminated waters, wildlife

Bacteriology HO (6 decks)

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