5c (Genetic Modification)

Question 1

Restriction Enzyme

Answer 1

An enzyme that cuts DNA at specific site to obtain desired gene and leaves sticky ends

Question 2

Ligase enzyme

Answer 2

An enzyme that forms bonds between Vector and Gene to join them together

Question 3

Recombinant DNA

Answer 3

DNA from 1 specie that also has DNA from another in the Human insulin story the new plasmid is recombinant

Question 4

Transgenic Organism

Answer 4

An organism which also has DNA from another species in Human insulin story the bacteria with the recombinant plasmid is a transgenic organism

Question 5

Vector

Answer 5

Moves DNA from one place to another. Plasmids or viruses can be used as vectors

Question 6

Human insuline

Answer 6

• Section of human DNA for growth hormone that makes insuline is cut out using restriction enzymes
• Plasmid that acts as vectors is taken out of bacteria and is cutted using same restriction enzymes
• Human gene for insuline was stuck into the plasmid using ligase enzymes
• Plasmid with the human insuline gene is put into a bacteria
• Fermenter is used to reproduce bacteria to produce much more human insuline