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Biology > 5.1 - Photosynthesis > Flashcards

5.1 - Photosynthesis Flashcards

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1
Q

Where do the light-dependent & light independent reactions occur in plants?

A
  • Light-dependent: in the thylakoids of chloroplasts
  • Light-independent: stroma of chloroplasts
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2
Q

Explain the role of light in photoionisation

A

Chlorophyll molecules absorb energy from photons of light. This ‘excites’ 2 electrons (raises them to a higher energy level), causing them to be released from the chrolophyll.

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3
Q

Name the 2 main stages involved in ATP production in the light-dependent reaction

A
  1. Electron transfer chain
  2. Chemiosmosis
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4
Q

What happens in the electron transfer chain (ETC)?

A

Electrons released from chlorophyll move down a series of carrier proteins embedded in the thylakoid membrane & undergo a series of redox reactions which releases energy.

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5
Q

How is a proton concentration gradient established during chemiosmosis?

A

Some energy is released from the ETC is coupled to the active transport of H+ ions (protons) from the stroma into the thylakoid space.

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6
Q

How does chemiosmosis produce ATP in the light-dependent stage?

A

H+ ions (protons) move down their concentration gradient from the thylakoid space into the stroma via the channel protein ATP synthase.
ATP synthase catalyses ADP + Pi –> ATP

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7
Q

Explain the role of light in photolysis

A

Light energy splits molecules of water 2H2O –> 4H+ + 4e- + O2

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8
Q

What happens to the products of the photolysis of water?

A
  • H+ ions: move out of thylakoid space via ATP synthase & are used to reduce the coenzyme NADP
  • e-: replace electrons lost from chlorophyll.
  • O2: used for respiration or diffuses out of leaf as waste gas.
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9
Q

How and where is reduced NADP produced in the light-dependent reaction?

A
  • NADP + 2H+ + 2e- —-> reduced NADP
  • Catalysed by dehydrogenase enzymes.
  • Stroma of chloroplasts
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10
Q

Where do the H+ ions and electrons used to reduce NADP come from?

A
  • H+ ions: photolysis of water
  • Electrons: NADP acts as the final electron acceptor of the electron transfer chain
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11
Q

Name 3 main stages in the Calvin cycle

A
  1. Carbon fixation
  2. Rduction
  3. Regeneration
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12
Q

What happen during carbon fixation?

A
  • Reaction between CO2 & ribulose biphosphate (RuBP) catalysed by rubisco.
  • Forms unstable 6C intermediate that breaks down into 2x glycerate 3-phosphate (GP).
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13
Q

What happens during reduction (in the Calvin cycle)?

A
  • 2 x GP are reduced to 2 x phosphate (TP)
  • Requires 2 x reduced NADP & 2x ATP
  • Forms 2 x NADP & 2 x ADP
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14
Q

What happens during regeneration (in the calvin cycle)?

A
  • After 1C leaves the cycle, the 5C coumpound RuP forms
  • RuBP is regenerated from RuP using 1 x ATP
  • Forms 1 x ADP
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15
Q

How does the light-independent reaction result in the production of useful organic substances?

A

1C leaves the cycle (i.e. some of the TP is converted into useful organic molecules).

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16
Q

State the roles of ATP & (reduced) NADP in the light-independent reaction.

A
  • ATP: redction of GP to TP & provides phosphate group to convert RuP into RuBP.
  • (reduced) NADP: coenzyme transports electrons needed for reduction of GP to TP.
17
Q

State the number of carbon atoms in RuBP, GP & TP.

A

RuBP: 5
GP: 3
TP: 3

18
Q

Describe the structure of a chloroplast.

A
  • Usually disc-shaped
  • Double membrane
  • Thylakoids: flattened discs stack to form grana
  • Intergranal lamellae: tubular extensions attach thylakoids in adjacent grana
  • Stroma: fluid-filled matrix
19
Q

How does the structure of the chloroplast maximise the rate of the light-dependent reaction?

A
  • ATP synthase channels within granal membrane.
  • Large surface area of thylakoid membrane for ETC.
  • Photosystems position chlorophyll to enable maximum absorbtion of light.
20
Q

How does the structure of chloroplast maximise the rate of the light-independent reaction?

A
  • Own DNA & ribosomes for synthesis of enzymes e.g. rubisco.
  • Concentration of enzymes & substrates in stroma is high.
21
Q

Define ‘limiting factor’.

A

Factor that determines maximum rate of a reaction, even if other factors change to become more favourable.

22
Q

Name 4 environmental factors that can limit the rate of photosynthesis.

A
  • Light intensity (light-dependent stage)
  • CO2 levels (light-independent stage)
  • Temperature (enzyme controlled steps)
  • Mineral/ magnesium levels (maintain normal functioning of chlorophyll)
23
Q

Outline some common agricultural practices used to overcome the effect of limiting factor in photosynthesis.

A
  • Artificial light, especially at night.
  • Artificial heating
  • Addition of CO2 to greenhouse atmosphere
24
Q

Why do farmers try to overcome the effect of limiting factors?

A
  • To increase yield
  • Additional cost must be balanced with yield to ensure maximun profit
25
Q

Suggest how a student could investigate the effect of a named variable on the rate of photosynthesis.

A

Dependent variable: rate of O2 production/ CO2 consumption
1. Use a potometer
2. Place balls of calcium alginate containing green algae in hydrogencarbonate indicator (colour change orange –> magenta as CO2 is consumed & pH increases)

26
Q

State the purpose and principle of paper chromatography

A

Molecules in a mixture are seperated based on their relative attraction to the mobile phase (running solvent) vs the stationary phase (chromatography paper).

27
Q

Outline a method for extractiong photosynthetic pigments

A

Use a pestle and mortar to grind a leaf with an extraction solvent e.g. propanone

28
Q

What are Rf values? How can they be calculated?

A
  • Ratios that allow comparison of how far molecules have moved in chromatograms
  • Rf value = distance between origin and centre of pigment spot/ distance between origin and solvent front.
28
Q

Outline a how paper chromatography can be used to seperate photosynthetic pigments.

A
  1. Use a capillary tube to spot pigment extract onto pencil ‘start time’ (origin) 1cm above bottom of paper.
  2. Place chromatography paper in solvent (origin should be above solvent level).
  3. Allow solvent to run until it almost touched the other end of the paper. Pigments move different distances.
29
Q

Can you explain how the plant leaf is adapted to carry our photosynthesis?

A
  • Large SA: absorb as much sunlight as possible
  • Arrangement of leaves: minimises overlapping and so avoids shadowing of on leaf over another
  • Thin: most light is absorbed in 1st few micrometres of lead + diffusion pathway distance for gases is kept short
  • Transparent cuticle and epidermis: let light through to p.s mesophyll cells beneath
  • Long, narrow upper mesophyll cells packed w/ chloroplasts: collect sunlight
  • Numerous stomata for gaseous exchange: all mesophyll cells are only a short diffusion pathway from one
  • Stomata that open and close: responds to changes in light intensity
  • Many air spaces: in lower mesophyll layer to allow rapid diffusion in gas phase of CO2 and O2
  • Network of xylem: brings water to leaf cells + phloem: carries away sugars produce during p.s
30
Q

Can you describe the main stages of photosynthesis?

A

1) Capturing of light energy: by chloroplast pigments such as chlorophyll
2) Light-dependent reaction: light energy absorbed is converted in chemical bonds. During process electron flow created by effect of light on chlorophyll, causing water to split (photolysis) into protons, electrons and oxygen. Products are reduced NADP, ATP and oxygen
3) Light-independent reaction: protons (H+) used to produce sugars and other organic molecules

31
Q

Can you explain the processes of oxidation and reduction?

A

OILRIG: electrons
Oxidation: loss of hydrogen or gain of oxygen
Reduction: gain H or lose O2

32
Q

Can you explain how ATP is made during the light-dependent reaction?

A
  1. Photoionisation of PSII
  2. Photolysis of water
  3. Photoionisation of PSI
  4. Active transport of H+ ions
  5. Oxidative phosphorylation
  6. Reducing NADP

Describe what happens during photoionisation of PSII
- Chlorophyll absorbs light
- Electrons excited and move to higher energy level
- Electrons move down electron transport chain to PSI

Describe what happens during photolysis of water
- Light energy splits water molecules into H+, e- and oxygen
- The electrons replace those lost from PSII during photoionisation

Explain how H+ ions are moved across the thylakoid membrane
- The excited electrons from photoionisation lose energy as they move down the electron transport chain
- The energy is used to actively

Explain how H+ are involved in the synthesis of ATP in chloroplasts
- H+ move down their concentration gradient into the stroma
- This is via ATP synthase in the thylakoid membrane
- The energy synthesises ATP from ADP and Pi

Explain why the photoionisation of PSI is important
- The excited eletrons are transferred to NADP with H+ to form reduced NADP
- This is needed for the light-independent reaction

What is chemiosmosis?
- Electrons move down the electron transport chain and creating a proton gradient
- This drives ATP synthesis

33
Q

Can you describe the role of photolysis in the light-dependent reaction?

A

Non-cyclic phosphorylation:
- Photoionisation of chlorophyll transfers excited electrons to electron acceptor
- Photolysis of H2O produces O2, H+ and electrons - e- transferred to positively-charged chlorophyll
- e- acceptor passes the electrons to the electron transport chain
- Further absorbed light energy increase the energy of the e-, sufficient for reduction of NADP+ to NADPH

34
Q

Can you explain how chloroplasts are adapted to carry out the light-dependent reaction?

A
  • Thylakoid membrane provides large SA for attachment of chlorophyll, electron carriers and enzymes that carry out light-depen reaction
  • Network of proteins in grana hold chlorophyll in precise manner that allows max absorption of light
  • Granal membranes have ATP synthase channels within them, which catalyse production of ATP. Selectively permeable membrane - allows estab. Of proton gradient
  • Contain both DNA and ribosomes; quickly and easily manufacture proteins involved in light-depen reaction
35
Q

Can you explain how carbon dioxide absorbed by plants is incorporated into organic molecules?

A
  • Carbon dioxide diffuses from the atmosphere into leaves through stomata and dissolves into water around the walls of the mesophyll cells and then into the stroma of the chloroplasts.
  • In the stroma the carbon dioxide reacts with RuBP catalysed with rubisco to produce GP (glycerate 3-phosphate)
  • Reduced NADP from the LDR is used to reduce GP to TP (triose phosphate) using energy supplied from ATP.
  • The NADP is re-formed and goes back to the LDR to be reduced again by accepting protons.
  • Some TP is converted into organic substances e.g. glucose.
  • Most TP is used to regenerate RuBP using ATP from the LDR.
36
Q

Can you describe the roles of ATP and reduced NADP in the light-independent reaction?

A

-The reduced NADP provides the reducing power (hydrogen) and is converted back to NADP which is then reduced again in the light-dependent reactions.
-ATP is also used to provide energy for the conversion. It is converted into ADP + Pi, which are reconverted into ATP in the light-dependent reactions.

37
Q

Can you describe the events of the Calvin cycle?

A

1) CO2 from atmo. Diffuses into leaf through stomata + dissolves in H2O around walls of meso. Cells. Then diffuses through cell-surface membrane, cytoplasm and chloroplast membranes into stroma of chloro.
2) In the stroma: CO2 reacts w/ 5C RuBP, the reaction catalysed by rubisco
3) This provides 2 molecules of 3C GP
4) Reduced NADP (NADPH) - from light dep- used to reduce GP to TP using energy supplied by ATP
5) NADP is re-formed and goes back light-dependent reaction to be reduced again by accepting more protons
6) Some TP molecules converted to organic substances that plant requires (starch, cellulose, lipids, glucose, amino acids, nucleotides)
7) Most TP used to regen RuBP using ATP from light-depen reaction

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