✅5: Photosynthesis Flashcards

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1
Q

What is an autotrophic organism

A

Makes complex organic compounds from simple compounds in their environment.

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2
Q

What is photosynthesis

A

Process by which living organisms capture the energy of the sun using chlorophyll and use it to covert co2 and water into simple sugars

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3
Q

What are heterotrophic organisms

A

Obtain complex molecules by feeding on other living organisms on their dead remains

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4
Q

What is the process of photosynthesis

A

Carbon dioxide and water goes to glucose and oxygen.

6co2 and 6h2o goes to c6h12o6 and 6o2,

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5
Q

What does the light do to the compounds?

A

The energy in the light is used to slit the strong H-O bonds in the water molecules. The hydrogen releases is combined with carbon dioxide to form fuel for the cells, glucose. Oxygen is releases in the atmosphere as a waste product.

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6
Q

Structure of the chloroplast

A

Relatively large organelles, green parts of the plant. Each chloroplast is surrounded by an outer and inner membrane with a space between the two called an envelope. Inside there is a system of membranes arranged in a stack called the GRANA. A single GRANUM is made up of stacks of membrane discs called THYLAKOIDS. This is where green pigment is. The grana are joined together by the LAMELLAE, extensions of the thylakoids membranes. The lamella acts as a skeleton within the chloroplast, these membrane stacks are surrounded my MATRIX. Called the STROMA.

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7
Q

Importance of chloroplast

A

The pigment molecules on the thylakoids are arranged on the membrane in the bets possible position to trap light energy. Evidence in light microscopes. Granal membranes are covering in particles that seen to involved ATP synthesis. The lamella maintains working distance between the grana so they get maximum ,igniting and function efficiently,

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8
Q

What do the stroma in chloroplast contain

A

Contain all enzymes needed to complete the process of photosynthesis and produce glucose.

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9
Q

What can glucose be used for

A

Cellular respiration, converted to starch for Storage, used as an intermediate for the synthesis of other organic compounds such as amino acids and lipids.

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10
Q

What is a chlorophyll

A

In the chloroplast, they are light capturing photosynthetic pigments. Involves chlorophyll A which is blue-green and chlorophyll B which is yellow green. Also carotenoids which are orange carotene and yellow. And phaephytin , which is a breakdown product of the others,

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11
Q

Where is chlorophyll A found

A

In all photosynthesising plants and is the most abundant of the 5.

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12
Q

Where are the other chlorophyll

A

Varying proportions , and it is these that causes different shades of green. Each pigment absorbs and captures light from particular areas of the spectrum.

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13
Q

What is the absorption spectrum?

A

Describes the range of amount of light of different wavelengths that a photosynthetic pigment absorbs. Usually represenated in a graph. The absorption spectra of the different photosynthetic pigments are found by measuring their absorption o flight of differing wavelengths.

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14
Q

How do you compare the rate of photosynthesis?

A

With the wavelength of light. Can use bacteria that move towards oxygen to show where the most oxygen is given off, which is directionally proportional to the rate of photosynthesis.

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15
Q

What is the action spectrum

A

Way of demonstrating the rate of photosynthesis against the wavelength of light

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16
Q

What is chromatography

A

With a suitable solvent, the pigments travel up the paper at different speeds and are readily separated, to show the different shades of green found in a plant.

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17
Q

How do you work our Rf values

A

Once you have carried out the chromatography, you can compare the Rf values to known values. Rf value is the ratio of distance travelled by the pigment to the distance travelled by the solvent alone.

Distance traveled by solute (pigment) % by the distance travelled by solvent.

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18
Q

What are photosystems?

A

The photosynthetic pigments carry out the absorption of light in 2 distinct chlorophyll complexes called PHOTOSYSTEM 1 and PHOTOSYSTEM II.

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19
Q

What do the photosystems contain

A

Different combination of chlorophyll pigments and so absorb light in a slightly different area of the spectrum.

20
Q

What are both of the photosystems

A

PSI particles are mainly on the intergranal lamellae.

PSII articles are on the grana themselves,

21
Q

What is the light dependent stage of photosynthesis?

A

Reactions that take place in the light on the thylakoids membranes of the chloroplast, they produce ATP and split water molecules in a photochemical reaction, providing hydrogen ions to reduce the carbon dioxide and produce carbohydrates,

22
Q

What is light?

A

Light is a form of electromagnetic radiation and the smallest unit of light is a photon.

23
Q

What happens when light hits a chlorophyll molcecule in light dependent photosynthesis

A

When a photon of light hits a chlorophyll molecule, the energy is transferred to the electrons of that molecule. The electrons get existed and are raised to higher energy levels. If an electron is raised to a sufficiently high energy level, it will leave the chlorophyll completely empty.

24
Q

What happens to the excited electron during light dependent photosynthesis

A

The excited electron can be picked up by an electron acceptor and result in the synthesis of ATP by cyclic phosphorylation and non cyclic.

25
Q

What is cyclic photophosphorylation

A

Process that drives the production of ATP. Light excited electrons from PS1 are taken up by an electron acceptor and passed directly along an ETC to produce ATP, with the electron returning to PS1. When the electron returns to the chlorophyll molecule in PS1, it can act in the same way again when the light comes again.

26
Q

What is non cyclic photophosphorylation

A

Process involving BOTH PSI and PSII. In which water moles are split using light energy to provide reducing power to make carbohydrates and at the same time produce more ATP.

27
Q

What is the shape of non cyclic photophosphorylation

A

An N shape. The hydrogen electron travels through PSII, and then down an ETC to the PSI, and up to an electron acceptor NADP. Light hits both photosystems, but the electron originally came form water.

28
Q

Why does PS1 in non cyclic p pick up the electron ?

A

To replace the one that was lost during light independent reactions.

29
Q

What is photolysis

A

Splitting of a molecule using light

30
Q

What are light independent stages of photosynthesis

A

Reactions that use the reduced NADP and ATo predicted by the light dependent stage of photosynthesis in a pathway called Calvin cycle. , which takes place in the stroma of the chloroplast and result in the reduction of CO2 from the air to bring about the synthesis of carbohydrates.

31
Q

What happens during non cyclic photophosphorylation

A

Light conditions: photons hit chlorophyll in PsI and PSII, excited electrons are lost and picked up by NADP. Excited electrom from PSII is picked up by another acceptor and passed along ETC, to PSI, driving ATP synthesis. PSI receives an electron.
Chlorophyll in PSII in short of electron and unstable. Original cannot be returned as it is at PSI! Therefore electron must be found somewhere to restore chlorophyll to its original state. Splitting of water by photolysis causes electron, H plus ions and hydroxide ions.
Replaces lost electrons.
Once PSII restored, ready to be excited again and its hit by ANTOHER photon of light,
Meanwhile, in PsI, also being excited by Keith, and lost to NADP, which also takes up dissociated water at same time.

32
Q

What does The produces of non cyclic photophosphorylation used for

A

As a source of reducing power and energy

33
Q

What happens in the calvin cycle?

Carbon fixation

A

This is first stage

co2 from air combines with ribulose bisphosphate 5 carbon compound. Co2 is fixed therefore called carbon fixation.

34
Q

What is a necssary compound in the first stage of the Calvin cycle?

A

The enzyme ribulose bisphosphate carboxylase (RUBSICO), is necessary for this step.

35
Q

What is the second stage of Calvin cycle?

A

The result of the reaction between ribulose bisphosphate and carbon dioxide is a 6 carbon compound, very unstable, and it immediately splits into 2 moles, giving:
Glycerate 3 phosphate. A 3 carbon compound.

36
Q

What is the third stage of Calvin cycle

A

Glycerate 3 phosphate is reduced (hydrogen is added) to form:
Glyceradehyde 3 phosphate (GALP) a 3 carbon sugar.

37
Q

Where does the hyd4en come from for glyceraldehyde 3 phosphate

A

Comes from reduced NADP, and the energy required comes from ATP, but produced in light dependent stage.

38
Q

What is the fourth stage of the Calvin cycle

A

The 3 carbon GALP passes through series of steps to replace ribulose bisphosphate needed for the first step of the Calvin cycle. However,
Some of it is synthesised into 6 carbon sugar glucose, or passed directly into glycolysis pathway for synthesis of other molecules.

39
Q

When does the Calvin cycle happen?

A

In the light and the dark. These can only stop when the products o flight reaction run our, leaving no reduced NADP or ATP available in the chloroplasts,

40
Q

What are the products of photosynthesis used for?

A

GALP is the primary end product and it is the key molecules for the synthesis of everything else needed for the plant. Some is passed directly to glycolysis and kerbs. Some is used to produce glucose in ‘gluconeogenesis’ reaction. This glucose may be converted into sucrose, for transport around plant, or staff as energy store, or cellulose, for structural support,
Also provide energy in the form of ATP.
Also for building blocks of amino acids, combining the molecules of nitrates from the soil.
Some is converted into acetyl coA which can synthesis fatty acids into production of phospholipids for membranes.

41
Q

What are alternative routes for photosynthesis?

A

The plants who use the normal way are called C3 plants, as the CO2 is fixed on 3 carbon compound.
Some plants have alternative ways of fixing carbon dioxide, and produce compound with 4 carbon atoms, to raise the levels of carbon dioxide. Known as C4 plants. Evolved in hot and dry ecosystems

42
Q

How is light a limiting factor of photosynthesis?

A

The amount of light available affects the amount of chlorophyll which is excited and therefore reduces the amount of reduced NADP and ATP produced in the night dependent stage.
If low levels of light, insufficient NaDp and ATP will be produced to allow the reactions of the light independent o progress and max rate, therefore limiting factor

43
Q

Why is carbon dioxide a limiting factor in photosynthesis

A

If not enough carbon dioxide, not enough for fixation in the Calvin cycle and the reaction cannot proceed at max rate. Carbon dioxide is most common limiting factor due to small levels in atmosphere.

44
Q

How to resolve carbon dioxide limiting factor

A

Greenhouses with carbon dioxide enriched atmosphere to increase production.

45
Q

How is temperature a limiting factor to photosynthesis

A

All of Calvin cycle and many of the light dependent reactions are controlled by enzymes, therefore temperature affects them. Even if light and co2 levels are good, unless temperature is good, plant will be unable to take advantage of conditions,

46
Q

What do plants vary in?

A

Nutrients. In the real world, plants need nutrients to convert carbohydrates into proteins and fats.