Brainscape's Knowledge GenomeTM

Browse over 1 million classes created by top students, professors, publishers, and experts.


See full index

FluoroFun > 5. class single molecules > Flashcards

5. class single molecules Flashcards

1
Q

Why doing single molecule detection ?

bulk experiment

A
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

Why doing single molecule detection ?

answer

A
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

Getting rid of ensemble averaging

A
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

No need for synchronization

A
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

The samples you want to look at:

A
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

How to do single molecule spectroscopy?

A
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

Technical considerations for SMD

A
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

How to do single molecule spectroscopy?

A
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

Scanning confocal vs wide field microscopy

A
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

Scanning confocal fluorescence microscopy

setup

A
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

Scanning confocal fluorescence microscopy

signal

A
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

Blinking of single molecules

A
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

Blinking of single molecules

jablonski

A
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

Two-color detection scheme: FRET

A
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

Two-color detection scheme: FRET

A
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
16
Q

Spectral measurements

A
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
17
Q

Life-time measurements

A
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
18
Q

macro micro time

A
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
19
Q

Single molecule lifetime measurements

A
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
20
Q

Single molecule lifetime measurements

A
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
21
Q

Sequential laser excitation schemes: PIE

A
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
22
Q

Wide-field microscopy

A
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
23
Q

Wide field microscopy

A
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
24
Q

Wide-field microscopy

A
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
25
Q

Wide-field microscopy

A
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
26
Q

Single particle tracking

A
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
27
Q
  1. SPT set-up
A
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
28
Q

1.1 SPT set-up: controlling laser exposure

A
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
29
Q

1.1 SPT set-up: controlling laser exposure

A
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
30
Q

1.1 SPT set-up: controlling laser exposure

A
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
31
Q

1.2 SPT set-up: matching image magnification to CCD pixel size

A
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
32
Q

1.2 SPT set-up: matching image magnification to CCD pixel size

A
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
33
Q

1.3 SPT set-up: Dual color SPT

A
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
34
Q

1.3 SPT set-up: Dual color SPT

A
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
35
Q
  1. Locating the particles by image processing
A
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
36
Q

2.1 Intensity thresholding - Examples

A
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
37
Q

2.2 Background removal

A
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
38
Q

2.3 Intensity thresholding - algorithms

A
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
39
Q

2.4 Object detection & selection

A
40
Q

2.5 Object properties

A
41
Q

2.7 SPT software – automated particle detection

A
42
Q
  1. Calculation of trajectories
A
43
Q

3.1 Nearest neighbour: caveats

A
44
Q

3.1 Nearest Neighbour: caveats

A
45
Q

3.2 Calculation of trajectories: examples

A
46
Q

4.1 Trajectory analysis - Theory

A
47
Q

Diffusion constants from the MSD ?

A
48
Q

4.2 Trajectory analysis – modes of motion

A
49
Q

4.3 Trajectory classification

A
50
Q

4.4 Localisation precision (Gaussian fit)

A
51
Q

4.5 Localisation precision determines lower mobility limit

A
52
Q

4.6 Trajectory analysis: example

A
53
Q
  1. SPT applications
A
54
Q

Application: intracellular trafficking of gene complexe

A
55
Q

Application: intracellular trafficking of gene complexes

A
56
Q

Advanced SPT

A
57
Q

Advanced orbital tracking

A
58
Q

Conclusions: SPT

A
59
Q

Single molecule enzymatics

A
60
Q

Single molecule enzymatics dynamics

A
61
Q

Time dependent kcat

A
62
Q

The lipase B from the yeast candida antarctica

A
63
Q

Non fluorescent substrate conversion

A
64
Q

Long observation time possible

A
65
Q

Several conformations are involved

A
66
Q

How many conformations are involved?

A
67
Q

The ‘lazy’ enzyme

A
68
Q

Size of the fluorogenic substrate

A
69
Q

Size of the substrate

A
70
Q

Single molecule enzymatics

A
71
Q

Single molecule enzymatics

A
72
Q

Single molecule enzymatics

A
73
Q

Phospholipases

A
74
Q

Phospholipases

A
75
Q

Single molecule enzymatics

A
76
Q

Single molecule enzymatics

first step

A

+ skript!

77
Q

Single molecule enzymatics?

A
78
Q

Dual color excitation, simultaneous detection

A
79
Q

Water soluble perylenediimide dye (PDI)

A
80
Q

Dual color excitation, simultaneous detection

A
81
Q

Active PLA1 – High concentration

A
82
Q

Active PLA1 – Low concentration

A
83
Q

Active PLA1 vs inactive

A
84
Q

Single enzyme trajectories

A
85
Q

Image analysis

A
86
Q

Wide field microscopy

A
87
Q

Wide-field microscopy

A
88
Q

Image analysis

A
89
Q

Cummulative distribution functions

A
90
Q

Enzyme mobility

A
91
Q

Model of mode of operation

A
92
Q

Getting closer to cell membranes….

A
93
Q

Getting closer to nature….

Hydrolysis of multi-component layers: POPC and DPG

A
94
Q

Hydrolysis of giant unilamellar vesicles (GUV’s)

A
95
Q

Hydrolysis of giant unilamellar vesicles (GUV’s)

A
96
Q

Cytoplasma ER

A
97
Q

YFP-PLCβ1 – Ca2+ response

A

FluoroFun (9 decks)

Brainscape helps you reach your goals faster, through stronger study habits.
© 2024 Bold Learning Solutions. Terms and Conditions