4. class labels Flashcards by Thomas Hasiweder

Q

content of this class

A

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Q

Natural fluorophores

A

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Organic fluorophores

Fluoresceins and rhodamines

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Q

Organic fluorophores

cyanine dyes

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Q

Alexa fluor dyes

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Photostability

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Q

Atto dyes

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Q

What are quantum dots?

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Q

How do quantum dots function?

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Properties of quantum dots

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Q

Quantum dot vs organic dye

pictures

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Quantum dot vs organic dye

wavelengths

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Q

Making quantum dots biocompatible

1 step

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Q

Making quantum dots biocompatible

2 step

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Q

QDs as nano carriers

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Q

Targetting cancer

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Q

Seeing cancer

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Q

Quantum dots

advantages vs. disadvantages

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Q

What are nano diamonds?

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Q

Properties of nanodiamonds

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Q

Nanodiamonds pictures

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Q

GFP

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backbone cyclization

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Q

Genetically encoded GFP

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Q

GFP applications

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Q

FP: spectral variants

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Q

DsRed

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Q

palette of FPs

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Indicator for cell cycle progression

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Q

photoswitchable FPs

types

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Q

photoswitchable FPs

mechanism

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photoconvertible GFP

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photoconvertible Kaede

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Q

FRET

basic concepts

A

Q

FRET based sensors

A

Q

single FP based sensor

A

Q

BiFC

A

Q

Fragmentizable proteins

A

Q

Essentials of BiFC

A

Q

BiFC

advantages & disadvantages

vs FRET

A

Q

Fluorophores for labeling

amines & thiols

A

Q

Fluorophores for labeling

click chemistry

A

Q

In vivo labelling:

the ideal method

A

Q

In vivo:

methods available

1-3

A

Q

In vivo:

methods available

4-5

A

Q

Antibodies

A

Q

HIV structure

virus

A

Q

HIV structure

fluorescence

A

Q

Nanobodies

A

Q

Fab fragments

A

Q

Immunostaining

advantages vs disadv.

A

Q

Fluorescent proteins

sequence

A

Q

FP

advantages & disadvantages

A

Q

cell division

A

Q

self labelling proteins

A

Q

mechanism halotag & snaptag

A

Q

mechanism clip tag

A

Q

labelling protocol

A

Q

Fluorescence imaging

A

Q

Selflabeling proteins

advantages & disadvantages

A

Q

Amber suppresion

A

Q

Protein synthesis

Reminder of the protein synthesis:

A

Q

Protein synthesis

trna

A

Q

amber suppresion

A

Q

Amber suppresion

examples

Genetic encoding and fluorogenic labeling of unnatural amino acid 1, 2 and 3

A

Q

Amber suppresion

examples

sitespecific

A

Q

Amber suppresion

advantages & disadvantages

A

Q

Selection guide

A

Q

CRISPR

A

Q

genomic manipulation

A

Q

genomic manipulation

gene editing

A

Q

genomic manipulation

Until recently, the existing approaches were based on modulated proteins

A

Q

genomic manipulation

originally from?

A

Q

genomic manipulation in bacteria

A

Q

genomic manipulation

scheme

A

Q

CRISPR nuclease complex

A

Q

CRISPR nuclease complex

bottom

A

Q

CRISPRS nuclease complex

One single plasmid has it all!

A

Q

genetic modifications

A

Q

labeling endogenous proteins

HMG

A

Q

labeling endogenous proteins

Vimentin

A

Q

labeling endogenous proteins

Zyxin

A

Q

in caenorhabditis

A

Q

CRISPR

advantages & disadvantages

A