10. Class: labelfree Flashcards by Thomas Hasiweder | Brainscape

Q

Optical microscopy in biology?

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content

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Molecular spectroscopy: matter

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Molecular spectroscopy: EM radiation

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Molecular spectroscopy: matter

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Light absorption and fluorescence

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Light absorption and fluorescence

transitions

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Resolution: diffraction limited

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Intrinsic fluorescence

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Fluorescence labeling

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Molecular spectroscopy: photon picture

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Vibrational energy

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(Spontaneous) Raman spectroscopy

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Raman vs Infrared spectrum

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Raman vs Infrared spectrum

wavelengths

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Raman vs Infrared spectrum

spectrum absorption

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Raman scattering intensity and shift

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Molecular spectroscopy: Light scattering

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a new type of secondary radiation

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Inelastic light scattering: Phenomenological

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Infrared spectroscopy / microscopy

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dimensions of biomolecules

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Raman micro-spectroscopy

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Raman microspectroscopy

advantages & disadvantages

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Raman µscopy: fixed lymphocytes

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Conclusion on Raman spectroscopy and comparison with infrared

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Stokes and anti-Stokes Raman scattering

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SERS: phenomenological

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Light induced Surface Plasmons

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Electric field enhancement: Antenna-effect

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Chemical enhancement

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SERS in cells

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Tip Enhanced RS (TERS)

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TERS for cell membrane analysis

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nonlinear?

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Two-Photon excitation

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Two-photon absorption

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Two-Photon excitation

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Two-Photon excitation => multiphoton

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Auto-fluorescence

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Pulsed lasers: time-averaged vs peak power!

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2nd harmonic generation

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2nd harmonic generation: what can we image?

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Drosophila larvae

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Nonlinear light-matter interactions

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Coherent Raman Imaging: CARS

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Coherent Raman Imaging: CARS

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Coherent Raman Imaging: CARS Setup for microscopy?

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Q

Nonlinear optical microscope

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Coherent Raman imaging

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Coherent Raman Imaging: CARS

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Drosophila polytene

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Small molecule labeling

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Small molecule labeling

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end

A