1. class Flashcards by Thomas Hasiweder

Q

Why fluorescence microscopy?

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Q

Why fluorescence microscopy?

GFP & quantum dots

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Q

Basics of fluorescence

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Q

Nature of light

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Visible light

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Light absorption

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Light absorption

text

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Q

Lambert-Beer Law

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Absorption and emission spectra

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Q

Simplified Jablonski diagram

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Asorption vs Fluorescence

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Stokes shift

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Jablonski diagram

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Q

Photophysical processes

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Q

Many things can happen…

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Q

Symmetry

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Q

Exceptions to the Mirror-Image Rule

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Q

Absorption and emission spectra

Quinine

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Q

Some history

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Q

Fluorescence quantum yield

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Q

Fluorescence lifetime

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Q

Fluorescence lifetime graph

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Q

Fluorescence lifetime

Eosin

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Q

Fluorescence lifetime

donor & acceptor

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Q

Intrinsic chromophores

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Extrinsic chromophores

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Q

Extrinsic chromophores

labeling

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Q

Light-matter interactions

absorption

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Luminescence incandescence

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Light-matter interactions

reflection & refraction

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Q

QUESTION: How do a perfectly diffuse or specular reflective surface look like?

A

Q

Measurement of absorption spectra

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Q

Measurement of fluorescence

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Measurement of fluorescence spectra

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Measurement of fluorescence quantum yield

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Measurement of fluorescence quantum yield

effects

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Measurement of lifetime

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Q

Measurement of lifetime

TAC reset:

A

Q

Measurement of lifetime

IRF

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Q

Example: CFP fluorescence decay

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Example: CFP fluorescence decay

heterogeneity

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Q

FLUORESCENCE ANISOTROPY

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Polarized light and photoselection

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photoselection

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Anisotropy experiment

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Sequence of events after excitation with polarized light

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Example: YFP

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Example: multichromophoric dendrimers

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Example: multichromophoric dendrimers

monomer vs dendrimer

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Magic angle

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Energy transfer

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Radiative energy transfer

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Energy transfer

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Förster Resonance Energy Transfer: FRET

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FRET

A

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FRET

R0

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FRET

ex & em

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Q

FRET

The critical transfer distance

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Q

FRET efficiency

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Some useful FRET numbers

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Q

Förster Resonance Energy Transfer: FRET

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Q

FRET can be used to study:

A

Q

Dexter energy transfer

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Q

Electron transfer

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Q

Electron transfer

photosynthesis

A

Q

Using Chlorophyl Fluorescence to Study Photosynthesis

A