1. class

Question 1

Why fluorescence microscopy?

Answer 1

Question 2

Why fluorescence microscopy?

GFP & quantum dots

Answer 2

Question 3

Basics of fluorescence

Answer 3

Question 4

Nature of light

Answer 4

Question 5

Visible light

Answer 5

Question 6

Light absorption

Answer 6

Question 7

Light absorption

text

Answer 7

Question 8

Lambert-Beer Law

Answer 8

Question 9

Absorption and emission spectra

Answer 9

Question 10

Simplified Jablonski diagram

Answer 10

Question 11

Asorption vs Fluorescence

Answer 11

Question 12

Stokes shift

Answer 12

Question 13

Jablonski diagram

Answer 13

Question 14

Photophysical processes

Answer 14

Question 15

Many things can happen…

Answer 15

Question 16

Symmetry

Answer 16

Question 17

Exceptions to the Mirror-Image Rule

Answer 17

Question 18

Absorption and emission spectra

Quinine

Answer 18

Question 19

Some history

Answer 19

Question 20

Fluorescence quantum yield

Answer 20

Question 21

Fluorescence lifetime

Answer 21

Question 22

Fluorescence lifetime graph

Answer 22

Question 23

Fluorescence lifetime

Eosin

Answer 23

Question 24

Fluorescence lifetime

donor & acceptor

Answer 24

Question 25

Intrinsic chromophores

Answer 25

Question 26

Extrinsic chromophores

Answer 26

Question 27

Extrinsic chromophores

labeling

Answer 27

Question 28

Light-matter interactions

absorption

Answer 28

Question 29

Luminescence incandescence

Answer 29

Question 30

Light-matter interactions

reflection & refraction

Answer 30

Question 31

QUESTION: How do a perfectly diffuse or specular reflective surface look like?

Answer 31

Question 32

Measurement of absorption spectra

Answer 32

Question 33

Measurement of fluorescence

Answer 33

Question 34

Measurement of fluorescence spectra

Answer 34

Question 35

Measurement of fluorescence quantum yield

Answer 35

Question 36

Measurement of fluorescence quantum yield

effects

Answer 36

Question 37

Measurement of lifetime

Answer 37

Question 38

Measurement of lifetime

TAC reset:

Answer 38

Question 39

Measurement of lifetime

IRF

Answer 39

Question 40

Example: CFP fluorescence decay

Answer 40

Question 41

Example: CFP fluorescence decay

heterogeneity

Answer 41

Question 42

FLUORESCENCE ANISOTROPY

Answer 42

Question 43

Polarized light and photoselection

Answer 43

Question 44

photoselection

Answer 44

Question 45

Anisotropy experiment

Answer 45

Question 46

Sequence of events after excitation with polarized light

Answer 46

Question 47

Example: YFP

Answer 47

Question 48

Example: multichromophoric dendrimers

Answer 48

Question 49

Example: multichromophoric dendrimers

monomer vs dendrimer

Answer 49

Question 50

Magic angle

Answer 50

Question 51

Energy transfer

Answer 51

Question 52

Radiative energy transfer

Answer 52

Question 53

Energy transfer

Answer 53

Question 54

Förster Resonance Energy Transfer: FRET

Answer 54

Question 55

FRET

Answer 55

Question 56

FRET

R0

Answer 56

Question 57

FRET

ex & em

Answer 57

Question 58

FRET

The critical transfer distance

Answer 58

Question 59

FRET efficiency

Answer 59

Question 60

Some useful FRET numbers

Answer 60

Question 61

Förster Resonance Energy Transfer: FRET

Answer 61

Question 62

FRET can be used to study:

Answer 62

Question 63

Dexter energy transfer

Answer 63

Question 64

Electron transfer

Answer 64

Question 65

Electron transfer

photosynthesis

Answer 65

Question 66

Using Chlorophyl Fluorescence to Study Photosynthesis

Answer 66