(5-11) Prokaryote Classification Flashcards

1. Define: Taxonomy, Identification, Classification, Nomenclature 2. Explain why organisms are arranged in taxonomic groups. 3. Define: Domain, Genus, Species, Strain 4. Describe the basis for the identification of prokaryotes using: Phenotypic characteristics, Genotypic characteristics 5. Describe the kind of information provided by: Morphological studies, Staining reactions, Biochemical reactions, Sequencing rRNA genes 6.Explain how rRNA sequences can be used in identification and classif

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1
Q

Why are living organisms divided into groups?

A

In order to better understand relationships among species.

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2
Q

What is Taxonomy?

A

The science that studies organisms to order and arrange them.

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3
Q

Which 3 areas can taxonomy be viewed in?

A
  1. Identification
  2. Classification
  3. Nomenclature
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4
Q

What is Identification?

A

Process of characterizing in order to group them

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5
Q

What is Classification?

A

Arranging organisms into similar or related groups

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6
Q

What is Nomenclature?

A

System of assigning names

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7
Q

Which 4 strategies are used to identify prokaryotes?

A
  1. Microscopic examination
  2. Culture characteristics
  3. Biochemical test
  4. Nucleic acid analysis
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8
Q

What are Taxonomic hierarchies?

A

Classification categories arranged in hierarchical order

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9
Q

What is the taxonomic hierarchy, from Domain to Strain?

A
  1. Domain
  2. (Kingdom)
  3. Phylum
  4. Class
  5. Order
  6. Family
  7. Genus
  8. Species
  9. Strain
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10
Q

Must different strains of the same species can have very similar characteristics? Give an example.

A
  1. No. Strains often have different characteristics.
  2. Ex. E. coli strain O157:H7 (a.ka., enterohemorrhagic E. coli, EHEC) causes bloody diarrhea and can lead to kidney failure and death.
    Another strain of E. coli (enterotoxigenic E. coli, ETEC) causes “traveler’s diarrhea.”
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11
Q

Which classification system do most microbiologists use?

A

Bergey’s Manual of Systematic Bacteriology

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12
Q

Which Classification scheme is favored by most biologists?

A

The 3 Domain system (as opposed to the 5 kingdom system)

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13
Q

Which code do microbiologists use for nomenclature?

A

Names are given according to International Code for the Nomenclature of Bacteria.

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14
Q

What is a dichotomous key?

A

A dichotomous key allows identification of prokaryotes using test that have a positive/negative (yes/no) result.

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15
Q

Which 4 tests might a dichotomous key use to identify a bacterium?

A
  1. Gram-stain
  2. Oxidase test
  3. Lactose fermentation
  4. Growth on citrate
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16
Q

What is the benefit of using genotype to identify prokaryotes?

A

One can Identify an organism that can’t be grown in culture.

17
Q

Why do we use sequencing ribosomal RNA genes (the DNA that encode rRNA) when using genotype to identify an organism?

A

There is little genetic variation in rRNA.

18
Q

Why is PCR used in identifying organismal genotype?

A

PCR is used to amplify sequences that allow for detection of specific sequences for identification.

19
Q

Who determined sequence of rRNA genes in wide variety of organisms.

A

Carl Woese

20
Q

How many major branches are prokaryotes divided into on the classification tree?

A

Two

21
Q

What does the length of line represent in the classification tree?

A

It represents the number of genetic differences between two organisms.